FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

Three-chromophore FRET microscopy to analyze multiprotein interactions in living cells.

Galperin E, Verkhusha VV, Sorkin A

Nearly every major process in a cell is carried out by assemblies of multiple dynamically interacting protein molecules. To study multi-protein interactions within such molecular machineries, we have developed a fluorescence microscopy method called three-chromophore fluorescence resonance energy transfer (3-FRET). This method allows analysis of three mutually dependent energy transfer processes between the fluorescent labels, such as cyan, yellow and ... [more]

Nat. Methods Dec. 01, 2004; 1(3);209-17 [Pubmed: 15782196]

Throughput

  • Low Throughput

Curated By

  • BioGRID