PARD3
Gene Ontology Biological Process
- asymmetric cell division [TAS]
- axonogenesis [TAS]
- cell junction assembly [TAS]
- cell-cell junction organization [TAS]
- establishment of epithelial cell polarity [ISS]
- establishment or maintenance of cell polarity [TAS]
- myelination in peripheral nervous system [ISS]
- negative regulation of peptidyl-threonine phosphorylation [ISS]
- positive regulation of myelination [ISS]
- protein complex assembly [TAS]
- protein kinase C-activating G-protein coupled receptor signaling pathway [TAS]
- protein targeting to membrane [ISS]
- tight junction assembly [ISS, TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
AMOT
Gene Ontology Biological Process
- actin cytoskeleton organization [TAS]
- cell-cell junction assembly [TAS]
- cellular protein localization [IDA]
- hippo signaling [IGI, TAS]
- negative regulation of angiogenesis [IDA]
- negative regulation of vascular permeability [IDA]
- positive regulation of blood vessel endothelial cell migration [IDA]
- positive regulation of cell size [TAS]
- positive regulation of stress fiber assembly [TAS]
- regulation of cell migration [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
A Rich1/Amot complex regulates the Cdc42 GTPase and apical-polarity proteins in epithelial cells.
Using functional and proteomic screens of proteins that regulate the Cdc42 GTPase, we have identified a network of protein interactions that center around the Cdc42 RhoGAP Rich1 and organize apical polarity in MDCK epithelial cells. Rich1 binds the scaffolding protein angiomotin (Amot) and is thereby targeted to a protein complex at tight junctions (TJs) containing the PDZ-domain proteins Pals1, Patj, ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| AMOT PARD3 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 36 | BioGRID | 2978844 | |
| PARD3 AMOT | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 78.75 | BioGRID | 2998372 |
Curated By
- BioGRID