LRRK2
Gene Ontology Biological Process
- GTP catabolic process [IDA]
- MAPK cascade [IDA]
- activation of MAPK activity [IDA]
- activation of MAPKK activity [IDA]
- cellular protein localization [ISS]
- cellular response to dopamine [IMP]
- cellular response to manganese ion [IMP]
- cellular response to oxidative stress [IMP]
- determination of adult lifespan [IMP]
- endocytosis [IGI, IMP]
- exploration behavior [IMP]
- intracellular distribution of mitochondria [IMP]
- intracellular signal transduction [ISS]
- negative regulation of GTPase activity [IDA]
- negative regulation of hydrogen peroxide-induced cell death [IMP]
- negative regulation of late endosome to lysosome transport [TAS]
- negative regulation of peroxidase activity [IDA]
- negative regulation of protein binding [IMP]
- negative regulation of protein phosphorylation [ISS]
- negative regulation of protein processing [IDA]
- negative regulation of protein processing involved in protein targeting to mitochondrion [IC]
- negative regulation of protein targeting to mitochondrion [IDA]
- negative regulation of thioredoxin peroxidase activity by peptidyl-threonine phosphorylation [IDA]
- neuromuscular junction development [IMP]
- neuron death [IMP]
- neuron projection morphogenesis [IMP]
- olfactory bulb development [IMP]
- peptidyl-serine phosphorylation [IDA]
- peptidyl-threonine phosphorylation [IDA, IMP]
- positive regulation of GTPase activity [IDA]
- positive regulation of MAP kinase activity [IC]
- positive regulation of autophagy [IMP, ISS]
- positive regulation of dopamine receptor signaling pathway [IMP]
- positive regulation of programmed cell death [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISS]
- positive regulation of protein autoubiquitination [IDA]
- positive regulation of protein binding [IDA]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of protein ubiquitination [IDA]
- protein autophosphorylation [IDA, IMP]
- protein localization to mitochondrion [TAS]
- protein phosphorylation [IMP]
- reactive oxygen species metabolic process [IMP]
- regulation of branching morphogenesis of a nerve [IMP]
- regulation of dendritic spine morphogenesis [IMP, ISS]
- regulation of dopamine receptor signaling pathway [ISS]
- regulation of excitatory postsynaptic membrane potential [ISS]
- regulation of kidney size [ISS]
- regulation of locomotion [IMP]
- regulation of membrane potential [IMP]
- regulation of mitochondrial depolarization [IMP]
- regulation of mitochondrial fission [TAS]
- regulation of neuroblast proliferation [IMP]
- regulation of neuron death [IMP]
- regulation of neuron maturation [IMP]
- regulation of protein kinase A signaling [ISS]
- regulation of synaptic transmission, glutamatergic [ISS]
- regulation of synaptic vesicle exocytosis [IMP]
- regulation of synaptic vesicle transport [ISS, TAS]
- response to oxidative stress [IMP]
- tangential migration from the subventricular zone to the olfactory bulb [IMP]
Gene Ontology Molecular Function- GTP binding [IDA]
- GTP-dependent protein kinase activity [IDA, IMP]
- GTPase activator activity [IDA]
- GTPase activity [IDA]
- MAP kinase kinase activity [IDA]
- Rho GTPase binding [IPI]
- SNARE binding [IPI]
- actin binding [IPI]
- clathrin binding [IPI]
- glycoprotein binding [IPI]
- identical protein binding [IPI]
- ion channel binding [IPI]
- kinase activity [IDA]
- peroxidase inhibitor activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- protein kinase A binding [IPI]
- protein kinase activity [IDA]
- protein serine/threonine kinase activity [IDA]
- syntaxin-1 binding [IPI]
- tubulin binding [IDA]
- GTP binding [IDA]
- GTP-dependent protein kinase activity [IDA, IMP]
- GTPase activator activity [IDA]
- GTPase activity [IDA]
- MAP kinase kinase activity [IDA]
- Rho GTPase binding [IPI]
- SNARE binding [IPI]
- actin binding [IPI]
- clathrin binding [IPI]
- glycoprotein binding [IPI]
- identical protein binding [IPI]
- ion channel binding [IPI]
- kinase activity [IDA]
- peroxidase inhibitor activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- protein kinase A binding [IPI]
- protein kinase activity [IDA]
- protein serine/threonine kinase activity [IDA]
- syntaxin-1 binding [IPI]
- tubulin binding [IDA]
Gene Ontology Cellular Component
- Golgi apparatus [ISS]
- axon [IDA, ISS]
- cytoplasm [IDA]
- cytoplasmic side of mitochondrial outer membrane [IDA]
- cytoplasmic vesicle [ISS]
- cytosol [IDA]
- dendrite [IDA, ISS]
- dendrite cytoplasm [IDA]
- endoplasmic reticulum [ISS]
- endosome [ISS]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- inclusion body [IMP]
- lysosome [ISS]
- mitochondrial inner membrane [ISS]
- mitochondrial matrix [ISS]
- mitochondrial membrane [IDA]
- mitochondrial outer membrane [ISS]
- mitochondrion [IDA]
- neuron projection [IDA]
- neuronal cell body [IDA]
- perikaryon [IDA, ISS]
- terminal bouton [TAS]
AGO2
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- Notch signaling pathway [TAS]
- RNA phosphodiester bond hydrolysis, endonucleolytic [EXP, IDA]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- gene expression [TAS]
- gene silencing by RNA [ISS]
- innate immune response [TAS]
- mRNA cleavage involved in gene silencing by miRNA [IDA, IMP]
- negative regulation of translation involved in gene silencing by miRNA [IDA, IMP]
- negative regulation of translational initiation [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [ISS]
- positive regulation of nuclear-transcribed mRNA poly(A) tail shortening [ISS]
- pre-miRNA processing [IDA]
- translation [NAS]
- translational initiation [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Identification of potential protein interactors of Lrrk2.
Pathogenic substitutions in the Lrrk2 protein have been shown to be an important cause of both familial and sporadic parkinsonism. The molecular pathway involved in Lrrk2 dopaminergic neuron degeneration remains elusive. Employing a combination of Lrrk2-mediated protein precipitation and tandem mass spectrometry, we identified 14 potential Lrrk2 binding partners. The majority of these interactions may be subgrouped into three functional ... [more]
Throughput
- Low Throughput
Ontology Terms
- hek-293t cell (BTO:0002181)
Additional Notes
- exogenous expression of bait
- table 1.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| LRRK2 AGO2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 722646 | |
| AGO2 LRRK2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 722647 |
Curated By
- BioGRID