Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The proximal signaling network of the BCR-ABL1 oncogene shows a modular organization.

Titz B, Low T, Komisopoulou E, Chen SS, Rubbi L, Graeber TG

BCR-ABL1 is a fusion tyrosine kinase, which causes multiple types of leukemia. We used an integrated proteomic approach that includes label-free quantitative protein complex and phosphorylation profiling by mass spectrometry to systematically characterize the proximal signaling network of this oncogenic kinase. The proximal BCR-ABL1 signaling network shows a modular and layered organization with an inner core of three leukemia transformation-relevant ... [more]

Oncogene Nov. 04, 2010; 29(44);5895-910 [Pubmed: 20697350]

Throughput

  • Low Throughput

Additional Notes

  • table S2.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CYFIP2 ABI1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
CYFIP2 ABI1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
2338375
CYFIP2 ABI1
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High0.8645BioGRID
2666365

Curated By

  • BioGRID