TNRC6C
Gene Ontology Biological Process
- embryonic hemopoiesis [IMP]
- embryonic organ development [IMP]
- endoderm development [IMP]
- endoderm formation [IMP]
- positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [ISO]
- positive regulation of nuclear-transcribed mRNA poly(A) tail shortening [ISO]
- regulation of gene silencing by miRNA [IMP]
AGO2
Gene Ontology Biological Process
- RNA phosphodiester bond hydrolysis, endonucleolytic [IDA, ISO]
- gene silencing by RNA [IMP]
- mRNA cleavage [IMP]
- mRNA cleavage involved in gene silencing by miRNA [ISO]
- miRNA metabolic process [IDA]
- negative regulation of translation involved in gene silencing by miRNA [ISO]
- negative regulation of translational initiation [ISO]
- positive regulation of gene expression [IMP]
- positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [IMP]
- positive regulation of nuclear-transcribed mRNA poly(A) tail shortening [IMP]
- post-embryonic development [IMP]
- pre-miRNA processing [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Ago-TNRC6 triggers microRNA-mediated decay by promoting two deadenylation steps.
MicroRNAs (miRNAs) silence the expression of their mRNA targets mainly by promoting mRNA decay. The mechanism, kinetics and participating enzymes for miRNA-mediated decay in mammalian cells remain largely unclear. Combining the approaches of transcriptional pulsing, RNA tethering, overexpression of dominant-negative mutants, and siRNA-mediated gene knockdown, we show that let-7 miRNA-induced silencing complexes (miRISCs), which contain the proteins Argonaute (Ago) and ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID