BAIT

CRYGC

CCL, CRYG3, CTRCT2
crystallin, gamma C
GO Process (1)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

HSPB1

CMT2F, HEL-S-102, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27
heat shock 27kDa protein 1
GO Process (21)
GO Function (7)
GO Component (8)
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Alteration of protein-protein interactions of congenital cataract crystallin mutants.

Fu L, Liang JJ

A recent study demonstrated the presence of protein-protein interactions among lens crystallins in a mammalian cell two-hybrid system assay and speculated about the significance of these interactions for protein solubility and lens transparency. The current study extends those findings to the following crystallin genes involved in some congenital cataracts: CRYAA (R116C), CRYAB (R120G), and CRYGC (T5P).A mammalian two-hybrid system was ... [more]

Invest. Ophthalmol. Vis. Sci. Mar. 01, 2003; 44(3);1155-9 [Pubmed: 12601044]

Throughput

  • Low Throughput

Additional Notes

  • mammalian two-hybrid system

Curated By

  • BioGRID