BAIT

HSPB1

CMT2F, HEL-S-102, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27
heat shock 27kDa protein 1
GO Process (21)
GO Function (7)
GO Component (8)
Homo sapiens
PREY

PHLDA1

DT1P1B11, PHRIP, TDAG51
pleckstrin homology-like domain, family A, member 1
GO Process (0)
GO Function (1)
GO Component (2)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A novel HSF1-mediated death pathway that is suppressed by heat shock proteins.

Hayashida N, Inouye S, Fujimoto M, Tanaka Y, Izu H, Takaki E, Ichikawa H, Rho J, Nakai A

Heat shock response is an adoptive response to proteotoxic stress, and a major heat shock transcription factor 1 (HSF1) has been believed to protect cells from cell death by inducing heat shock proteins (Hsps) that assist protein folding and prevent protein denaturation. However, it is revealed recently that HSF1 also promotes cell death of male germ cells. Here, we found ... [more]

EMBO J. Oct. 18, 2006; 25(20);4773-83 [Pubmed: 17024176]

Throughput

  • Low Throughput

Curated By

  • BioGRID