BAIT

CRYAB

CMD1II, CRYA2, CTPP2, CTRCT16, HEL-S-101, HSPB5, MFM2
crystallin, alpha B
Alzheimer's Disease Project
UPS Project
Homo sapiens
PREY

FCGR2A

CD32, CD32A, CDw32, FCG2, FCGR2, FCGR2A1, FcGR, IGFR2, RP11-5K23.6
Fc fragment of IgG, low affinity IIa, receptor (CD32)
GO Process (2)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Chaperone activity of α B-crystallin is responsible for its incorrect assignment as an autoantigen in multiple sclerosis.

Rothbard JB, Zhao X, Sharpe O, Strohman MJ, Kurnellas M, Mellins ED, Robinson WH, Steinman L

For 15 y, α B-crystallin (heat shock protein [Hsp] B5) has been labeled an autoantigen in multiple sclerosis (MS) based on humoral and cellular responses found in humans and animal models. However, there have been several scientific inconsistencies with this assignment, ranging from studies demonstrating small differences in anticrystallin responses between patients and healthy individuals to the inability of crystallin-specific ... [more]

J. Immunol. Apr. 01, 2011; 186(7);4263-8 [Pubmed: 21357544]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
FCGR2A CRYAB
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-

Curated By

  • BioGRID