BAIT

FIS1

TTC11, CGI-135
fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Homo sapiens
PREY

CASP8

ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5
caspase 8, apoptosis-related cysteine peptidase
GO Process (33)
GO Function (9)
GO Component (10)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Fis1 and Bap31 bridge the mitochondria-ER interface to establish a platform for apoptosis induction.

Iwasawa R, Mahul-Mellier AL, Datler C, Pazarentzos E, Grimm S

The mitochondria and the endoplasmic reticulum (ER) are two organelles that critically contribute to apoptosis induction. While it is established that they communicate, how cell death signals are transmitted from the mitochondria to the ER is unknown. Here, we show that the mitochondrial fission protein Fission 1 homologue (Fis1) conveys an apoptosis signal from the mitochondria to the ER by ... [more]

EMBO J. Feb. 02, 2011; 30(3);556-68 [Pubmed: 21183955]

Throughput

  • Low Throughput

Curated By

  • BioGRID