GNB4
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GNAI2
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [TAS]
- adenosine receptor signaling pathway [IBA]
- adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway [IBA, TAS]
- blood coagulation [TAS]
- cell division [IMP]
- gamma-aminobutyric acid signaling pathway [IBA]
- negative regulation of adenylate cyclase activity [TAS]
- platelet activation [TAS]
- response to nutrient [TAS]
- signal transduction [TAS]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
FRET
An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.
Publication
Gialpha and Gbeta subunits both define selectivity of G protein activation by alpha2-adrenergic receptors.
Previous studies of the specificity of receptor interactions with G protein subunits in living cells have relied on measurements of second messengers or other downstream responses. We have examined the selectivity of interactions between alpha2-adrenergic receptors (alpha2R) and various combinations of Gialpha and Gbeta subunit isoforms by measuring changes in FRET between Gialpha-yellow fluorescent protein and cyan fluorescent protein-Gbeta chimeras ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GNAI2 GNB4 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9998 | BioGRID | 1186610 | |
| GNAI2 GNB4 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9943 | BioGRID | 2238577 | |
| GNAI2 GNB4 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9716 | BioGRID | 3107792 |
Curated By
- BioGRID