BAG1
Gene Ontology Biological Process
- chaperone cofactor-dependent protein refolding [ISO]
- negative regulation of apoptotic process [IMP, ISO]
- negative regulation of motor neuron apoptotic process [ISO]
- negative regulation of protein phosphorylation [ISO]
- neuron differentiation [ISO]
- positive regulation of neuron apoptotic process [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- protein localization to mitochondrion [ISO]
- response to drug [IEP]
- response to lithium ion [IEP]
- response to nicotine [IEP]
- response to organic cyclic compound [IEP]
Gene Ontology Molecular Function
RAF1
Gene Ontology Biological Process
- MAPK cascade [IDA, IMP]
- activation of MAPKK activity [IDA, IMP, ISO, TAS]
- cell differentiation [ISO]
- death-inducing signaling complex assembly [ISO]
- heart development [IEP]
- intermediate filament cytoskeleton organization [ISO]
- intracellular signal transduction [TAS]
- negative regulation of apoptotic process [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors [ISO]
- negative regulation of protein complex assembly [ISO]
- neurotrophin TRK receptor signaling pathway [ISO]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- protein phosphorylation [IDA, IMP, TAS]
- response to hypoxia [IDA]
- response to muscle stretch [ISO]
- somatic stem cell maintenance [ISO]
Gene Ontology Molecular Function- ATP binding [IDA]
- MAP kinase kinase kinase activity [IDA, IMP, TAS]
- Ras GTPase binding [ISO]
- identical protein binding [ISO]
- mitogen-activated protein kinase kinase binding [IMP]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein serine/threonine kinase activity [ISO]
- small GTPase binding [ISO]
- ATP binding [IDA]
- MAP kinase kinase kinase activity [IDA, IMP, TAS]
- Ras GTPase binding [ISO]
- identical protein binding [ISO]
- mitogen-activated protein kinase kinase binding [IMP]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein serine/threonine kinase activity [ISO]
- small GTPase binding [ISO]
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- cytoplasm [IDA, ISO]
- cytosol [ISO, TAS]
- plasma membrane [IDA, ISO]
- pseudopodium [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
BAG-1 proteins protect cardiac myocytes from simulated ischemia/reperfusion-induced apoptosis via an alternate mechanism of cell survival independent of the proteasome.
BAG-1 (Bcl-2-associated athanogene-1) proteins interact with the HSC70 and HSP70 heat shock proteins and have been proposed to promote cell survival by coordinating the function of these chaperones with the proteasome to facilitate protein degradation. Consistent with this proposal, previous analyses in cancer cells have demonstrated that BAG-1 requires protein domains important for HSC70/HSP70 and proteasome binding in order to ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID