Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Bypass of glycan-dependent glycoprotein delivery to ERAD by up-regulated EDEM1.

Ron E, Shenkman M, Groisman B, Izenshtein Y, Leitman J, Lederkremer GZ

Trimming of mannose residues from the N-linked oligosaccharide precursor is a stringent requirement for glycoprotein endoplasmic reticulum (ER)-associated degradation (ERAD). In this paper, we show that, surprisingly, overexpression of ER degradation-enhancing α-mannosidase-like protein 1 (EDEM1) or its up-regulation by IRE1, as occurs in the unfolded protein response, overrides this requirement and renders unnecessary the expression of ER mannosidase I. An ... [more]

Mol. Biol. Cell Nov. 01, 2011; 22(21);3945-54 [Pubmed: 21917589]

Throughput

  • Low Throughput

Curated By

  • BioGRID