BAIT

BAG3

BAG-3, BIS, CAIR-1, MFM6
BCL2-associated athanogene 3
Alzheimer's Disease Project
GO Process (3)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

CRYAB

AACRYA
crystallin, alpha B
GO Process (21)
GO Function (7)
GO Component (14)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Rattus norvegicus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

BAG3 directly interacts with mutated alphaB-crystallin to suppress its aggregation and toxicity.

Hishiya A, Salman MN, Carra S, Kampinga HH, Takayama S

A homozygous disruption or genetic mutation of the bag3 gene causes progressive myofibrillar myopathy in mouse and human skeletal and cardiac muscle disorder while mutations in the small heat shock protein αB-crystallin gene (CRYAB) are reported to be responsible for myofibrillar myopathy. Here, we demonstrate that BAG3 directly binds to wild-type αB-crystallin and the αB-crystallin mutant R120G, via the intermediate ... [more]

PLoS ONE Mar. 23, 2011; 6(3);e16828 [Pubmed: 21423662]

Throughput

  • Low Throughput

Curated By

  • BioGRID