Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The ubiquitin specific protease-4 (USP4) interacts with the S9/Rpn6 subunit of the proteasome.

Zhao B, Velasco K, Sompallae R, Pfirrmann T, Masucci MG, Lindsten K

The proteasome is the major non-lysosomal proteolytic machine in cells that, through degradation of ubiquitylated substrates, regulates virtually all cellular functions. Numerous accessory proteins influence the activity of the proteasome by recruiting or deubiquitylating proteasomal substrates, or by maintaining the integrity of the complex. Here we show that the ubiquitin specific protease (USP)-4, a deubiquitylating enzyme with specificity for both ... [more]

Biochem. Biophys. Res. Commun. Oct. 26, 2012; 427(3);490-6 [Pubmed: 23022198]

Throughput

  • Low Throughput

Curated By

  • BioGRID