ITGA1
Gene Ontology Biological Process
- axon guidance [TAS]
- blood coagulation [TAS]
- cell-matrix adhesion [IMP]
- extracellular matrix organization [TAS]
- muscle contraction [TAS]
- negative regulation of cell proliferation [IMP]
- negative regulation of epidermal growth factor receptor signaling pathway [ISS]
- positive regulation of phosphoprotein phosphatase activity [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SHARPIN
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
SHARPIN is an endogenous inhibitor of β1-integrin activation.
Regulated activation of integrins is critical for cell adhesion, motility and tissue homeostasis. Talin and kindlins activate β1-integrins, but the counteracting inhibiting mechanisms are poorly defined. We identified SHARPIN as an important inactivator of β1-integrins in an RNAi screen. SHARPIN inhibited β1-integrin functions in human cancer cells and primary leukocytes. Fibroblasts, leukocytes and keratinocytes from SHARPIN-deficient mice exhibited increased β1-integrin ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
ITGA1 SHARPIN | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID