An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Targeting of KRAS mutant tumors by HSP90 inhibitors involves degradation of STK33.

Azoitei N, Hoffmann CM, Ellegast JM, Ball CR, Obermayer K, Goessele U, Koch B, Faber K, Genze F, Schrader M, Kestler HA, Doehner H, Chiosis G, Glimm H, Froehling S, Scholl C

Previous efforts to develop drugs that directly inhibit the activity of mutant KRAS, the most commonly mutated human oncogene, have not been successful. Cancer cells driven by mutant KRAS require expression of the serine/threonine kinase STK33 for their viability and proliferation, identifying STK33 as a context-dependent therapeutic target. However, specific strategies for interfering with the critical functions of STK33 are ... [more]

J. Exp. Med. Apr. 09, 2012; 209(4);697-711 [Pubmed: 22451720]

Throughput

High Throughput

Ontology Terms

cell line: mda-mb-231 cell (BTO:0000815) [adenocarcinoma (DOID:299)]
cell line: bt-20 cell (BTO:0001466)

Additional Notes

exogenous expression of bait

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
STK33 USP15	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Azoitei N (2012)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

STK33

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein serine/threonine kinase activity [ISS]

USP15

Gene Ontology Biological Process

Gene Ontology Molecular Function

SMAD binding [IPI]cysteine-type endopeptidase activity [IMP]identical protein binding [IPI]protein binding [IPI]transforming growth factor beta receptor binding [IPI]ubiquitin thiolesterase activity [IDA]ubiquitin-specific protease activity [IDA]ubiquitinated histone binding [IDA]

Gene Ontology Cellular Component

Affinity Capture-MS