BAIT
GRK5
GPRK5, RP11-714M16.1
G protein-coupled receptor kinase 5
GO Process (5)
GO Function (4)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
MADD
DENN, IG20, RAB3GEP
MAP-kinase activating death domain
GO Process (9)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
- activation of MAPK activity [IMP]
- cell surface receptor signaling pathway [TAS]
- execution phase of apoptosis [IMP]
- positive regulation of Rab GTPase activity [IDA, IMP]
- regulation of Rab protein signal transduction [IDA]
- regulation of apoptotic process [IMP]
- regulation of cell cycle [IMP]
- regulation of extrinsic apoptotic signaling pathway [IDA]
- regulation of extrinsic apoptotic signaling pathway via death domain receptors [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Targeted Ubiquitination and Degradation of G-Protein-Coupled Receptor Kinase 5 by the DDB1-CUL4 Ubiquitin Ligase Complex.
The G protein-coupled receptor kinases (GRKs) phosphorylate agonist occupied G protein-coupled receptors (GPCRs) and desensitize GPCR-mediated signaling. Recent studies indicate they also function non-catalytically via interaction with other proteins. In this study, a proteomic approach was used to screen interacting proteins of GRK5 in MDA-MB-231 cells and HUVEC cells. Mass spectrometry analysis reveals several proteins in the GRK5 immunocomplex including ... [more]
PLoS ONE Sep. 07, 2012; 7(8);e43997 [Pubmed: 22952844]
Throughput
- High Throughput
Curated By
- BioGRID