BAIT
GRK5
GPRK5, RP11-714M16.1
G protein-coupled receptor kinase 5
GO Process (5)
GO Function (4)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ARF1
ADP-ribosylation factor 1
GO Process (16)
GO Function (4)
GO Component (5)
Gene Ontology Biological Process
- COPI coating of Golgi vesicle [TAS]
- GTP catabolic process [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- cellular copper ion homeostasis [IMP]
- dendritic spine organization [ISS]
- long term synaptic depression [ISS]
- membrane organization [TAS]
- phosphatidylinositol biosynthetic process [TAS]
- phospholipid metabolic process [TAS]
- post-Golgi vesicle-mediated transport [TAS]
- regulation of Arp2/3 complex-mediated actin nucleation [ISS]
- regulation of defense response to virus by virus [TAS]
- regulation of receptor internalization [ISS]
- retrograde vesicle-mediated transport, Golgi to ER [TAS]
- small molecule metabolic process [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Targeted Ubiquitination and Degradation of G-Protein-Coupled Receptor Kinase 5 by the DDB1-CUL4 Ubiquitin Ligase Complex.
The G protein-coupled receptor kinases (GRKs) phosphorylate agonist occupied G protein-coupled receptors (GPCRs) and desensitize GPCR-mediated signaling. Recent studies indicate they also function non-catalytically via interaction with other proteins. In this study, a proteomic approach was used to screen interacting proteins of GRK5 in MDA-MB-231 cells and HUVEC cells. Mass spectrometry analysis reveals several proteins in the GRK5 immunocomplex including ... [more]
PLoS ONE Sep. 07, 2012; 7(8);e43997 [Pubmed: 22952844]
Throughput
- High Throughput
Curated By
- BioGRID