SMAD2
Gene Ontology Biological Process
- SMAD protein complex assembly [IDA]
- activin receptor signaling pathway [IMP]
- anterior/posterior pattern specification [ISS]
- cell fate commitment [ISS]
- common-partner SMAD protein phosphorylation [IDA]
- gastrulation [TAS]
- gene expression [TAS]
- intracellular signal transduction [ISS]
- mesoderm formation [ISS]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of transcription, DNA-templated [IMP]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- nodal signaling pathway [IMP]
- palate development [ISS]
- paraxial mesoderm morphogenesis [ISS]
- positive regulation of BMP signaling pathway [IMP]
- positive regulation of epithelial to mesenchymal transition [ISS]
- positive regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, ISS, TAS]
- positive regulation of transcription, DNA-templated [IDA, IMP, ISS]
- primary miRNA processing [TAS]
- regulation of binding [ISS]
- regulation of transforming growth factor beta receptor signaling pathway [IMP]
- response to cholesterol [IDA]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [IDA, IMP, TAS]
- zygotic specification of dorsal/ventral axis [IMP]
Gene Ontology Molecular Function- DNA binding [IDA]
- I-SMAD binding [IPI]
- R-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- SMAD binding [IPI]
- activating transcription factor binding [IPI]
- co-SMAD binding [IPI]
- double-stranded DNA binding [ISS]
- enhancer binding [IC]
- phosphatase binding [IPI]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- transforming growth factor beta receptor binding [IPI]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [IDA]
- type I transforming growth factor beta receptor binding [IPI]
- ubiquitin protein ligase binding [IPI]
- DNA binding [IDA]
- I-SMAD binding [IPI]
- R-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- SMAD binding [IPI]
- activating transcription factor binding [IPI]
- co-SMAD binding [IPI]
- double-stranded DNA binding [ISS]
- enhancer binding [IC]
- phosphatase binding [IPI]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- transforming growth factor beta receptor binding [IPI]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [IDA]
- type I transforming growth factor beta receptor binding [IPI]
- ubiquitin protein ligase binding [IPI]
Gene Ontology Cellular Component
SMAD1
Gene Ontology Biological Process
- BMP signaling pathway [IDA, IMP, ISS, TAS]
- SMAD protein complex assembly [IDA]
- cellular response to BMP stimulus [ISS]
- embryonic pattern specification [ISS]
- positive regulation of gene expression [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IC, IDA]
- positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus [ISS]
- primary miRNA processing [TAS]
- signal transduction [NAS]
- transforming growth factor beta receptor signaling pathway [NAS]
Gene Ontology Molecular Function- I-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- co-SMAD binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- receptor signaling protein activity [NAS]
- sequence-specific DNA binding transcription factor activity [IDA]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [TAS]
- I-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- co-SMAD binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- receptor signaling protein activity [NAS]
- sequence-specific DNA binding transcription factor activity [IDA]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [TAS]
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Identification of novel Smad2 and Smad3 associated proteins in response to TGF-beta1.
Transforming growth factor-beta 1 (TGF-beta1) is an important growth inhibitor of epithelial cells and insensitivity to this cytokine results in uncontrolled cell proliferation and can contribute to tumorigenesis. TGF-beta1 signals through the TGF-beta type I and type II receptors, and activates the Smad pathway via phosphorylation of Smad2 and Smad3. Since little is known about the selective activation of Smad2 ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SMAD1 SMAD2 | FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins. | High | - | BioGRID | 2641269 | |
| SMAD1 SMAD2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| SMAD2 SMAD1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID