HUS1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- nucleoplasm [TAS]
- nucleus [IDA]
MSH3
Gene Ontology Biological Process
- ATP catabolic process [IBA]
- DNA repair [IDA]
- maintenance of DNA repeat elements [IMP]
- meiotic mismatch repair [IBA]
- mismatch repair [IMP]
- negative regulation of DNA recombination [IDA]
- positive regulation of helicase activity [IDA]
- reciprocal meiotic recombination [IBA]
- somatic recombination of immunoglobulin gene segments [IBA]
Gene Ontology Molecular Function- DNA-dependent ATPase activity [IBA]
- Y-form DNA binding [IBA]
- dinucleotide insertion or deletion binding [IDA]
- dinucleotide repeat insertion binding [IDA]
- double-strand/single-strand DNA junction binding [IBA]
- enzyme binding [IPI]
- guanine/thymine mispair binding [IDA]
- heteroduplex DNA loop binding [IBA]
- mismatched DNA binding [IDA]
- oxidized purine DNA binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- single guanine insertion binding [IDA]
- single-stranded DNA binding [IDA]
- DNA-dependent ATPase activity [IBA]
- Y-form DNA binding [IBA]
- dinucleotide insertion or deletion binding [IDA]
- dinucleotide repeat insertion binding [IDA]
- double-strand/single-strand DNA junction binding [IBA]
- enzyme binding [IPI]
- guanine/thymine mispair binding [IDA]
- heteroduplex DNA loop binding [IBA]
- mismatched DNA binding [IDA]
- oxidized purine DNA binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- single guanine insertion binding [IDA]
- single-stranded DNA binding [IDA]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Interaction between human mismatch repair recognition proteins and checkpoint sensor Rad9-Rad1-Hus1.
In eukaryotic cells, the cell cycle checkpoint proteins Rad9, Rad1, and Hus1 form the 9-1-1 complex which is structurally similar to the proliferating cell nuclear antigen (PCNA) sliding clamp. hMSH2/hMSH6 (hMutS alpha) and hMSH2/hMSH3 (hMutS beta) are the mismatch recognition factors of the mismatch repair pathway. hMutS alpha has been shown to physically and functionally interact with PCNA. Moreover, DNA ... [more]
Throughput
- Low Throughput
Related interactions
Curated By
- BioGRID