PREY

ITCH

ADMFD, AIF4, AIP4, NAPP1, dJ468O1.1
itchy E3 ubiquitin protein ligase
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

HIV-1 Nef impairs heterotrimeric G-protein signaling by targeting Gαi2 for degradation through Ubiquitinylation.

Chandrasekaran P, Buckley M, Moore V, Wang LQ, Kehrl JH, Venkatesan S

The human immune deficiency (HIV) Nef protein is an important pathogenic factor that modulates cell surface receptor trafficking and impairs cell motility presumably by interfering at multiple steps with chemotactic receptor signaling. Here, we report that a dominant effect of Nef is to trigger AIP4 E3 ligase mediated Gαi2 ubiquitinylation, which leads to Gαi2 endolysosomal sequestration and destruction. The loss ... [more]

J. Biol. Chem. Oct. 15, 2012; 0(0); [Pubmed: 23071112]

Throughput

  • Low Throughput

Curated By

  • BioGRID