BAIT

VPS54

HCC8, PPP1R164, SLP-8p, VPS54L, WR, hVps54L
vacuolar protein sorting 54 homolog (S. cerevisiae)
GO Process (2)
GO Function (2)
GO Component (4)
Homo sapiens
PREY

VPS53

HCCS1, PCH2E, hVps53L, PP13624
vacuolar protein sorting 53 homolog (S. cerevisiae)
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Ang2/Fat-free Is a Conserved Subunit of the Golgi-associated Retrograde Protein (GARP) Complex.

Perez-Victoria FJ, Schindler C, Magadan JG, Mardones GA, Delevoye C, Romao M, Raposo G, Bonifacino JS

Monitoring Editor: Adam Linstedt The Golgi-associated Retrograde Protein (GARP) complex mediates tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN). In the yeast S. cerevisiae, GARP comprises four subunits named Vps51p, Vps52p, Vps53p and Vps54p. Orthologues of the GARP subunits, except for Vps51p, have been identified in all other eukaryotes. A yeast two-hybrid screen of a human ... [more]

Unknown Aug. 04, 2010; 0(0); [Pubmed: 20685960]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
VPS53 VPS54
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
VPS54 VPS53
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
VPS53 VPS54
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1BioGRID
2218096
VPS53 VPS54
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1BioGRID
3096489

Curated By

  • BioGRID