An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Ang2/Fat-free Is a Conserved Subunit of the Golgi-associated Retrograde Protein (GARP) Complex.

Perez-Victoria FJ, Schindler C, Magadan JG, Mardones GA, Delevoye C, Romao M, Raposo G, Bonifacino JS

Monitoring Editor: Adam Linstedt The Golgi-associated Retrograde Protein (GARP) complex mediates tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN). In the yeast S. cerevisiae, GARP comprises four subunits named Vps51p, Vps52p, Vps53p and Vps54p. Orthologues of the GARP subunits, except for Vps51p, have been identified in all other eukaryotes. A yeast two-hybrid screen of a human ... [more]

Unknown Aug. 04, 2010; 0(0); [Pubmed: 20685960]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

VAMP4

Gene Ontology Biological Process

Gene Ontology Molecular Function

SNAP receptor activity [IBA]SNARE binding [IBA]

Gene Ontology Cellular Component

VPS53