BAIT
DRD2
dopamine receptor D2
GO Process (99)
GO Function (11)
GO Component (15)
Gene Ontology Biological Process
- G-protein coupled receptor internalization [IDA]
- G-protein coupled receptor signaling pathway [ISO]
- Wnt signaling pathway [IDA]
- acid secretion [IDA]
- activation of protein kinase activity [IDA]
- adenohypophysis development [ISO]
- adenylate cyclase-inhibiting dopamine receptor signaling pathway [IDA, ISO]
- adenylate cyclase-modulating G-protein coupled receptor signaling pathway [IDA]
- adult behavior [ISO]
- adult walking behavior [ISO]
- arachidonic acid secretion [ISO]
- associative learning [ISO]
- auditory behavior [IMP]
- axonogenesis [ISO]
- behavioral response to cocaine [ISO]
- behavioral response to ethanol [ISO]
- branching morphogenesis of a nerve [ISO]
- cellular potassium ion homeostasis [IC]
- cerebral cortex GABAergic interneuron migration [ISO]
- circadian regulation of gene expression [ISO]
- dopamine metabolic process [ISO]
- dopamine receptor signaling pathway [IDA, IMP, ISO, TAS]
- feeding behavior [ISO]
- forebrain development [IMP]
- grooming behavior [ISO]
- intracellular signal transduction [ISO]
- locomotory behavior [IMP, ISO]
- long-term memory [IMP]
- negative regulation of adenylate cyclase activity [IDA, ISO]
- negative regulation of blood pressure [ISO]
- negative regulation of cell migration [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of circadian sleep/wake cycle, sleep [IDA]
- negative regulation of cytosolic calcium ion concentration [IMP]
- negative regulation of dopamine receptor signaling pathway [ISO]
- negative regulation of dopamine secretion [IMP]
- negative regulation of insulin secretion [IMP]
- negative regulation of protein kinase B signaling [ISO]
- negative regulation of protein secretion [ISO]
- negative regulation of synaptic transmission, glutamatergic [ISO]
- negative regulation of voltage-gated calcium channel activity [IDA, ISO]
- neurological system process involved in regulation of systemic arterial blood pressure [ISO]
- neuron-neuron synaptic transmission [ISO]
- orbitofrontal cortex development [IEP]
- peristalsis [ISO]
- phosphatidylinositol metabolic process [IDA]
- phospholipase C-activating dopamine receptor signaling pathway [ISO]
- pigmentation [ISO]
- positive regulation of ERK1 and ERK2 cascade [IMP]
- positive regulation of G-protein coupled receptor protein signaling pathway [IMP]
- positive regulation of cytokinesis [ISO]
- positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway [ISO]
- positive regulation of dopamine uptake involved in synaptic transmission [ISO]
- positive regulation of growth hormone secretion [ISO]
- positive regulation of long-term synaptic potentiation [IMP]
- positive regulation of mitosis [TAS]
- positive regulation of multicellular organism growth [ISO]
- positive regulation of neuroblast proliferation [ISO]
- positive regulation of receptor internalization [IMP]
- positive regulation of renal sodium excretion [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of urine volume [ISO]
- prepulse inhibition [ISO]
- protein localization [ISO]
- regulation of MAPK cascade [IDA]
- regulation of cAMP metabolic process [ISO]
- regulation of dopamine secretion [ISO]
- regulation of dopamine uptake involved in synaptic transmission [ISO]
- regulation of heart rate [ISO]
- regulation of locomotion involved in locomotory behavior [IMP]
- regulation of long-term neuronal synaptic plasticity [ISO]
- regulation of phosphoprotein phosphatase activity [IMP]
- regulation of potassium ion transport [IDA, ISO]
- regulation of sodium ion transport [ISO]
- regulation of synapse structural plasticity [IDA]
- regulation of synaptic transmission [IDA, ISO]
- regulation of synaptic transmission, GABAergic [ISO]
- release of sequestered calcium ion into cytosol [IMP]
- response to amphetamine [IEP, ISO]
- response to axon injury [IEP]
- response to cocaine [ISO]
- response to drug [IDA, ISO]
- response to ethanol [IEP]
- response to histamine [ISO]
- response to hypoxia [IMP]
- response to inactivity [IEP]
- response to iron ion [IEP]
- response to light stimulus [ISO]
- response to morphine [ISO]
- response to nicotine [IEP]
- response to toxic substance [IEP]
- sensory perception of smell [ISO]
- startle response [ISO]
- striatum development [IEP]
- synapse assembly [IEP]
- synaptic transmission [ISO]
- synaptic transmission, dopaminergic [IDA, IMP]
- temperature homeostasis [ISO]
- visual learning [ISO]
Gene Ontology Molecular Function- G-protein coupled receptor activity [ISO]
- dopamine binding [IMP, ISO]
- dopamine neurotransmitter receptor activity [IMP, ISO]
- dopamine neurotransmitter receptor activity, coupled via Gi/Go [IDA, ISO]
- drug binding [IDA, ISO]
- identical protein binding [ISO]
- ionotropic glutamate receptor binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IDA]
- receptor binding [IPI]
- G-protein coupled receptor activity [ISO]
- dopamine binding [IMP, ISO]
- dopamine neurotransmitter receptor activity [IMP, ISO]
- dopamine neurotransmitter receptor activity, coupled via Gi/Go [IDA, ISO]
- drug binding [IDA, ISO]
- identical protein binding [ISO]
- ionotropic glutamate receptor binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IDA]
- receptor binding [IPI]
Gene Ontology Cellular Component
- acrosomal vesicle [IDA]
- axon [IDA, ISO]
- axon terminus [IDA]
- cytoplasmic vesicle [IDA]
- dendrite [IDA, ISO]
- dendritic spine [IDA]
- endocytic vesicle [IDA]
- integral component of plasma membrane [ISO]
- lateral plasma membrane [IDA]
- membrane [IDA, ISO]
- perikaryon [IDA]
- plasma membrane [IDA]
- postsynaptic density [IDA]
- sperm flagellum [IDA]
- synaptic vesicle membrane [IDA]
Rattus norvegicus
PREY
CALM1
CaMI, rCG_20808
calmodulin 1
GO Process (21)
GO Function (19)
GO Component (14)
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [ISO]
- activation of adenylate cyclase activity [IDA]
- calcium-mediated signaling [IMP]
- detection of calcium ion [ISO]
- positive regulation of DNA binding [ISO]
- positive regulation of cyclic nucleotide metabolic process [ISO]
- positive regulation of cyclic-nucleotide phosphodiesterase activity [ISO]
- positive regulation of nitric-oxide synthase activity [IDA]
- positive regulation of phosphoprotein phosphatase activity [ISO]
- positive regulation of protein dephosphorylation [ISO]
- positive regulation of ryanodine-sensitive calcium-release channel activity [ISO]
- regulation of cardiac muscle contraction [ISO]
- regulation of cytokinesis [ISO]
- regulation of heart rate [ISO]
- regulation of high voltage-gated calcium channel activity [IMP]
- regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum [ISO]
- regulation of ryanodine-sensitive calcium-release channel activity [IDA]
- regulation of store-operated calcium channel activity [IC]
- response to amphetamine [IEP]
- response to calcium ion [ISO]
- substantia nigra development [ISO]
Gene Ontology Molecular Function- N-terminal myristoylation domain binding [ISO]
- adenylate cyclase binding [IDA]
- calcium channel regulator activity [NAS]
- calcium ion binding [IDA, ISO]
- calcium-dependent protein binding [IPI]
- enzyme regulator activity [IMP]
- ion channel binding [IDA, ISO]
- nitric-oxide synthase binding [IDA]
- nitric-oxide synthase regulator activity [IDA]
- phosphatidylinositol 3-kinase binding [IPI]
- phospholipase binding [ISO]
- protein N-terminus binding [IMP]
- protein binding [IPI]
- protein domain specific binding [IPI, ISO]
- protein kinase binding [ISO]
- protein phosphatase activator activity [ISO]
- thioesterase binding [ISO]
- titin binding [ISO]
- type 3 metabotropic glutamate receptor binding [IPI]
- N-terminal myristoylation domain binding [ISO]
- adenylate cyclase binding [IDA]
- calcium channel regulator activity [NAS]
- calcium ion binding [IDA, ISO]
- calcium-dependent protein binding [IPI]
- enzyme regulator activity [IMP]
- ion channel binding [IDA, ISO]
- nitric-oxide synthase binding [IDA]
- nitric-oxide synthase regulator activity [IDA]
- phosphatidylinositol 3-kinase binding [IPI]
- phospholipase binding [ISO]
- protein N-terminus binding [IMP]
- protein binding [IPI]
- protein domain specific binding [IPI, ISO]
- protein kinase binding [ISO]
- protein phosphatase activator activity [ISO]
- thioesterase binding [ISO]
- titin binding [ISO]
- type 3 metabotropic glutamate receptor binding [IPI]
Gene Ontology Cellular Component
Rattus norvegicus
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
Interactions between calmodulin, adenosine A2A, and dopamine D2 receptors.
The Ca(2+)-binding protein calmodulin (CaM) has been shown to bind directly to cytoplasmic domains of some G protein-coupled receptors, including the dopamine D(2) receptor. CaM binds to the N-terminal portion of the long third intracellular loop of the D(2) receptor, within an Arg-rich epitope that is also involved in the binding to G(i/o) proteins and to the adenosine A(2A) receptor, ... [more]
J. Biol. Chem. Oct. 09, 2009; 284(41);28058-68 [Pubmed: 19632986]
Throughput
- Low Throughput
Curated By
- BioGRID