BAIT

GRB2

ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2
growth factor receptor-bound protein 2
Homo sapiens
PREY

DNM3

Dyna III, RP1-106H8.3
dynamin 3
GO Process (4)
GO Function (2)
GO Component (4)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Charting the molecular network of the drug target Bcr-Abl.

Brehme M, Hantschel O, Colinge J, Kaupe I, Planyavsky M, Koecher T, Mechtler K, Bennett KL, Superti-Furga G

The tyrosine kinase Bcr-Abl causes chronic myeloid leukemia and is the cognate target of tyrosine kinase inhibitors like imatinib. We have charted the protein-protein interaction network of Bcr-Abl by a 2-pronged approach. Using a monoclonal antibody we have first purified endogenous Bcr-Abl protein complexes from the CML K562 cell line and characterized the set of most tightly-associated interactors by MS. ... [more]

Proc. Natl. Acad. Sci. U.S.A. May. 05, 2009; 106(18);7414-9 [Pubmed: 19380743]

Throughput

  • High Throughput

Ontology Terms

  • cell line: k-562 cell (BTO:0000664) [chronic myeloid leukemia (DOID:8552)]

Additional Notes

  • TAP-tagged Grb2
  • exogenous expression of bait

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GRB2 DNM3
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
832547
GRB2 DNM3
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1BioGRID
2220406
GRB2 DNM3
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1BioGRID
3129532
GRB2 DNM3
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-

Curated By

  • BioGRID