Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Charting the molecular network of the drug target Bcr-Abl.

Brehme M, Hantschel O, Colinge J, Kaupe I, Planyavsky M, Koecher T, Mechtler K, Bennett KL, Superti-Furga G

The tyrosine kinase Bcr-Abl causes chronic myeloid leukemia and is the cognate target of tyrosine kinase inhibitors like imatinib. We have charted the protein-protein interaction network of Bcr-Abl by a 2-pronged approach. Using a monoclonal antibody we have first purified endogenous Bcr-Abl protein complexes from the CML K562 cell line and characterized the set of most tightly-associated interactors by MS. ... [more]

Proc. Natl. Acad. Sci. U.S.A. May. 05, 2009; 106(18);7414-9 [Pubmed: 19380743]

Throughput

  • High Throughput

Ontology Terms

  • cell line: k-562 cell (BTO:0000664) [chronic myeloid leukemia (DOID:8552)]

Additional Notes

  • TAP-tagged CRK
  • exogenous expression of bait

Curated By

  • BioGRID