An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Charting the molecular network of the drug target Bcr-Abl.

Brehme M, Hantschel O, Colinge J, Kaupe I, Planyavsky M, Koecher T, Mechtler K, Bennett KL, Superti-Furga G

The tyrosine kinase Bcr-Abl causes chronic myeloid leukemia and is the cognate target of tyrosine kinase inhibitors like imatinib. We have charted the protein-protein interaction network of Bcr-Abl by a 2-pronged approach. Using a monoclonal antibody we have first purified endogenous Bcr-Abl protein complexes from the CML K562 cell line and characterized the set of most tightly-associated interactors by MS. ... [more]

Proc. Natl. Acad. Sci. U.S.A. May. 05, 2009; 106(18);7414-9 [Pubmed: 19380743]

Throughput

High Throughput

Ontology Terms

cell line: k-562 cell (BTO:0000664) [chronic myeloid leukemia (DOID:8552)]

Additional Notes

TAP-tagged AP2M1
exogenous expression of bait

Curated By

BioGRID

Interaction Summary

AP2M1

Gene Ontology Biological Process

Gene Ontology Molecular Function

clathrin adaptor activity [TAS]low-density lipoprotein particle receptor binding [ISS]protein binding [IPI]signal sequence binding [IDA]transporter activity [TAS]

Gene Ontology Cellular Component

HSPA9

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]ubiquitin protein ligase binding [IPI]unfolded protein binding [TAS]