An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The human core exosome interacts with differentially localized processive RNases: hDIS3 and hDIS3L.

Tomecki R, Kristiansen MS, Lykke-Andersen S, Chlebowski A, Larsen KM, Szczesny RJ, Drazkowska K, Pastula A, Andersen JS, Stepien PP, Dziembowski A, Jensen TH

The eukaryotic RNA exosome is a ribonucleolytic complex involved in RNA processing and turnover. It consists of a nine-subunit catalytically inert core that serves a structural function and participates in substrate recognition. Best defined in Saccharomyces cerevisiae, enzymatic activity comes from the associated subunits Dis3p (Rrp44p) and Rrp6p. The former is a nuclear and cytoplasmic RNase II/R-like enzyme, which possesses ... [more]

EMBO J. Jul. 21, 2010; 29(14);2342-57 [Pubmed: 20531386]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
DIS3L GIGYF2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Hein MY (2015)	High	-	BioGRID	1452676
DIS3L GIGYF2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Marcon E (2014)	High	29.5421	BioGRID	2944372

Curated By

BioGRID

Interaction Summary

DIS3L

Gene Ontology Biological Process

Gene Ontology Molecular Function

3'-5'-exoribonuclease activity [IDA, IMP]enzyme binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

GIGYF2