SMAD3
Gene Ontology Biological Process
- SMAD protein complex assembly [IPI, ISO]
- SMAD protein signal transduction [ISO]
- T cell activation [ISO]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway [IMP]
- activin receptor signaling pathway [ISO]
- cell cycle arrest [ISO]
- cell-cell junction organization [ISO]
- developmental growth [ISO]
- embryonic cranial skeleton morphogenesis [ISO]
- embryonic foregut morphogenesis [ISO]
- embryonic pattern specification [ISO]
- endoderm development [ISO]
- evasion or tolerance of host defenses by virus [ISO]
- extrinsic apoptotic signaling pathway [ISO]
- gastrulation [ISO]
- heart looping [ISO]
- immune response [ISO]
- immune system development [ISO]
- in utero embryonic development [ISO]
- intracellular signal transduction [ISO]
- lens fiber cell differentiation [ISO]
- liver development [ISO]
- mesoderm formation [ISO]
- negative regulation of apoptotic process [IDA]
- negative regulation of cell growth [ISO]
- negative regulation of cell proliferation [IDA]
- negative regulation of inflammatory response [ISO]
- negative regulation of mitotic cell cycle [ISO]
- negative regulation of osteoblast differentiation [ISO]
- negative regulation of osteoblast proliferation [ISO]
- negative regulation of protein catabolic process [ISO]
- negative regulation of protein phosphorylation [ISO]
- negative regulation of transcription from RNA polymerase II promoter [IMP, ISO]
- negative regulation of wound healing [ISO]
- nodal signaling pathway [ISO]
- osteoblast development [ISO]
- osteoblast differentiation [ISO]
- paraxial mesoderm morphogenesis [ISO]
- pericardium development [ISO]
- positive regulation of alkaline phosphatase activity [IDA]
- positive regulation of bone mineralization [IDA]
- positive regulation of canonical Wnt signaling pathway [ISO]
- positive regulation of catenin import into nucleus [ISO]
- positive regulation of cell migration [IMP]
- positive regulation of chondrocyte differentiation [ISO]
- positive regulation of epithelial to mesenchymal transition [ISO]
- positive regulation of focal adhesion assembly [IDA]
- positive regulation of gene expression [ISO]
- positive regulation of interleukin-1 beta production [IMP]
- positive regulation of positive chemotaxis [IDA]
- positive regulation of stress fiber assembly [IDA]
- positive regulation of transcription factor import into nucleus [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP, ISO]
- positive regulation of transcription, DNA-templated [ISO]
- positive regulation of transforming growth factor beta3 production [IMP]
- protein stabilization [ISO]
- regulation of binding [ISO]
- regulation of epithelial cell proliferation [ISO]
- regulation of immune response [ISO]
- regulation of striated muscle tissue development [ISO]
- regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of transforming growth factor beta receptor signaling pathway [ISO]
- regulation of transforming growth factor beta2 production [ISO]
- response to hypoxia [ISO]
- signal transduction involved in regulation of gene expression [IMP]
- skeletal system development [ISO]
- somitogenesis [ISO]
- thyroid gland development [ISO]
- transdifferentiation [IDA]
- transforming growth factor beta receptor signaling pathway [IMP, ISO]
- transport [ISO]
Gene Ontology Molecular Function- DNA binding [IDA]
- R-SMAD binding [ISO]
- RNA polymerase II activating transcription factor binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [ISO]
- SMAD binding [IPI]
- bHLH transcription factor binding [ISO]
- beta-catenin binding [ISO]
- chromatin DNA binding [ISO]
- chromatin binding [ISO]
- co-SMAD binding [ISO]
- collagen binding [ISO]
- core promoter proximal region sequence-specific DNA binding [ISO, ISS]
- double-stranded DNA binding [ISO]
- enzyme binding [IPI]
- identical protein binding [ISO]
- phosphatase binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- protein homodimerization activity [ISO]
- protein kinase binding [ISO]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription factor binding [IDA, IPI, ISO]
- transcription regulatory region DNA binding [ISO]
- transforming growth factor beta receptor binding [ISO]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [ISO]
- ubiquitin binding [ISO]
- ubiquitin protein ligase binding [ISO]
- zinc ion binding [ISO]
- DNA binding [IDA]
- R-SMAD binding [ISO]
- RNA polymerase II activating transcription factor binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [ISO]
- SMAD binding [IPI]
- bHLH transcription factor binding [ISO]
- beta-catenin binding [ISO]
- chromatin DNA binding [ISO]
- chromatin binding [ISO]
- co-SMAD binding [ISO]
- collagen binding [ISO]
- core promoter proximal region sequence-specific DNA binding [ISO, ISS]
- double-stranded DNA binding [ISO]
- enzyme binding [IPI]
- identical protein binding [ISO]
- phosphatase binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- protein homodimerization activity [ISO]
- protein kinase binding [ISO]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription factor binding [IDA, IPI, ISO]
- transcription regulatory region DNA binding [ISO]
- transforming growth factor beta receptor binding [ISO]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [ISO]
- ubiquitin binding [ISO]
- ubiquitin protein ligase binding [ISO]
- zinc ion binding [ISO]
Gene Ontology Cellular Component
AKT1
Gene Ontology Biological Process
- activation-induced cell death of T cells [ISO]
- aging [IDA]
- apoptotic mitochondrial changes [ISO]
- cell projection organization [IDA]
- cellular response to epidermal growth factor stimulus [ISO]
- cellular response to granulocyte macrophage colony-stimulating factor stimulus [ISO]
- cellular response to growth factor stimulus [ISO]
- cellular response to hypoxia [IDA]
- cellular response to insulin stimulus [IMP, ISO]
- cellular response to mechanical stimulus [IDA]
- cellular response to organic cyclic compound [IDA]
- cellular response to peptide [ISO]
- execution phase of apoptosis [ISO]
- germ cell development [ISO]
- glucose homeostasis [ISO]
- glucose metabolic process [ISO]
- glucose transport [ISO]
- glycogen biosynthetic process [IMP]
- glycogen cell differentiation involved in embryonic placenta development [ISO]
- glycogen metabolic process [ISO]
- hyaluronan metabolic process [IEP]
- inflammatory response [ISO]
- insulin receptor signaling pathway [IMP, ISO, ISS]
- insulin-like growth factor receptor signaling pathway [ISO, ISS]
- intracellular signal transduction [ISO]
- labyrinthine layer blood vessel development [ISO]
- maternal placenta development [ISO]
- negative regulation of JNK cascade [IDA]
- negative regulation of apoptotic process [ISO, TAS]
- negative regulation of autophagy [ISO]
- negative regulation of cell size [IDA]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO, ISS]
- negative regulation of endopeptidase activity [ISO]
- negative regulation of fatty acid beta-oxidation [ISO]
- negative regulation of plasma membrane long-chain fatty acid transport [ISO]
- negative regulation of protein kinase activity [IDA, ISO]
- negative regulation of proteolysis [ISO]
- negative regulation of release of cytochrome c from mitochondria [ISO, ISS]
- osteoblast differentiation [ISO]
- peptidyl-serine phosphorylation [IDA, ISO]
- peripheral nervous system myelin maintenance [ISO]
- phosphorylation [ISO, ISS]
- positive regulation of apoptotic process [IMP]
- positive regulation of blood vessel endothelial cell migration [ISO]
- positive regulation of cell growth [IDA, ISO]
- positive regulation of cellular protein metabolic process [IMP]
- positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle [ISO]
- positive regulation of endothelial cell proliferation [ISO, ISS]
- positive regulation of establishment of protein localization to plasma membrane [ISO]
- positive regulation of fat cell differentiation [ISO]
- positive regulation of glucose import [ISO]
- positive regulation of glucose metabolic process [ISO]
- positive regulation of glycogen biosynthetic process [ISO]
- positive regulation of lipid biosynthetic process [ISO]
- positive regulation of nitric oxide biosynthetic process [ISO]
- positive regulation of nitric-oxide synthase activity [ISO]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [ISO]
- positive regulation of sodium ion transport [IMP, ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of vasoconstriction [IMP]
- protein catabolic process [ISO]
- protein import into nucleus, translocation [ISO]
- protein kinase B signaling [ISO]
- protein phosphorylation [IDA, ISO, ISS]
- protein ubiquitination [ISO]
- regulation of cell migration [IMP, ISO]
- regulation of glycogen biosynthetic process [ISO]
- regulation of myelination [ISO]
- regulation of neuron projection development [ISO, ISS]
- regulation of protein localization [IMP, ISO]
- response to UV-A [ISO]
- response to fluid shear stress [ISO]
- response to food [ISO]
- response to hormone [ISO]
- response to oxidative stress [ISS]
- signal transduction [IDA]
- striated muscle cell differentiation [ISO]
- translation [IMP]
Gene Ontology Molecular Function- 14-3-3 protein binding [ISO]
- ATP binding [IDA, ISO]
- enzyme binding [IPI]
- identical protein binding [ISO]
- kinase activity [ISO]
- nitric-oxide synthase regulator activity [ISO]
- phosphatidylinositol-3,4,5-trisphosphate binding [ISO]
- phosphatidylinositol-3,4-bisphosphate binding [ISO]
- protein binding [IPI]
- protein kinase C binding [IPI]
- protein kinase activity [IDA, ISO]
- protein kinase binding [ISO]
- protein phosphatase 2A binding [IPI]
- protein serine/threonine kinase activity [IDA, ISO, ISS, TAS]
- protein serine/threonine/tyrosine kinase activity [ISO]
- 14-3-3 protein binding [ISO]
- ATP binding [IDA, ISO]
- enzyme binding [IPI]
- identical protein binding [ISO]
- kinase activity [ISO]
- nitric-oxide synthase regulator activity [ISO]
- phosphatidylinositol-3,4,5-trisphosphate binding [ISO]
- phosphatidylinositol-3,4-bisphosphate binding [ISO]
- protein binding [IPI]
- protein kinase C binding [IPI]
- protein kinase activity [IDA, ISO]
- protein kinase binding [ISO]
- protein phosphatase 2A binding [IPI]
- protein serine/threonine kinase activity [IDA, ISO, ISS, TAS]
- protein serine/threonine/tyrosine kinase activity [ISO]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Novel roles of Akt and mTOR in suppressing TGF-beta/ALK5-mediated Smad3 activation.
Insulin-like growth factor-I inhibits transforming growth factor-beta (TGF-beta) signaling by blocking activation of Smad3 (S3), via a phosphatidylinositol 3-kinase (PI3K)/Akt-dependent pathway. Here we provide the first report that the kinase activity of Akt is necessary for its ability to suppress many TGF-beta responses, including S3 activation and induction of apoptosis. Wild-type and myristoylated Akts (Akt(WT) and Akt(Myr)) suppress TGF-beta-induced phospho-activation ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID