An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Alpha-synuclein promotes SNARE-complex assembly in vivo and in vitro.

Burre J, Sharma M, Tsetsenis T, Buchman V, Etherton MR, Suedhof TC

Presynaptic nerve terminals release neurotransmitters repeatedly, often at high frequency, and in relative isolation from neuronal cell bodies. Repeated release requires cycles of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)-complex assembly and disassembly, with continuous generation of reactive SNARE-protein intermediates. Although many forms of neurodegeneration initiate presynaptically, only few pathogenic mechanisms are known, and the functions of presynaptic proteins linked ... [more]

Science Sep. 24, 2010; 329(5999);1663-7 [Pubmed: 20798282]

Throughput

Low Throughput

Additional Notes

figure 1.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SNCA SNAP25	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Burre J (2010)	Low	-	BioGRID	727278
SNCA SNAP25	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Sharma M (2011)	Low	-	BioGRID	-
SNAP25 SNCA	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Sharma M (2011)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SNAP25

Gene Ontology Biological Process

Gene Ontology Molecular Function

SNAP receptor activity [IBA]SNARE binding [IDA, ISO]calcium-dependent protein binding [ISO]myosin binding [ISO]protein N-terminus binding [ISO]protein binding [IPI]protein domain specific binding [ISO]syntaxin-1 binding [IBA, ISO]voltage-gated potassium channel activity [ISO]

Gene Ontology Cellular Component

SNCA