BAIT

RB1

OSRC, PPP1R130, RB, p105-Rb, pRb, pp110, RP11-174I10.1
retinoblastoma 1
GO Process (24)
GO Function (11)
GO Component (6)
Homo sapiens
PREY

RBAK

ZNF769
RB-associated KRAB zinc finger
GO Process (2)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Cloning and characterization of a novel Kruppel-associated box family transcriptional repressor that interacts with the retinoblastoma gene product, RB.

Skapek SX, Jansen D, Wei TF, McDermott T, Huang W, Olson EN, Lee EY

The retinoblastoma gene product, RB, seems to function as a key tumor suppressor by repressing the expression of genes activated by members of the E2F family of transcription factors. In order to accomplish this, RB has been proposed to interact with a transcriptional repressor. However, no genuine transcriptional repressors have been identified by virtue of interaction with RB. By using ... [more]

J. Biol. Chem. Mar. 10, 2000; 275(10);7212-23 [Pubmed: 10702291]

Throughput

  • Low Throughput

Curated By

  • BioGRID