BAIT

SPRY1

hSPRY1
sprouty homolog 1, antagonist of FGF signaling (Drosophila)
GO Process (2)
GO Function (1)
GO Component (2)
Homo sapiens
PREY

CBLB

Cbl-b, RNF56, Nbla00127
Cbl proto-oncogene B, E3 ubiquitin protein ligase
GO Process (2)
GO Function (1)
GO Component (4)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Recruitment of Sprouty1 to immune synapse regulates T cell receptor signaling.

Lee JS, Lee JE, Oh YM, Park JB, Choi H, Choi CY, Kim IH, Lee SH, Choi K

TCR stimulation not only initiates positive signals for T cell activation, but also induces negative signals that down-regulate T cells. We previously reported that Sprouty1, a negative regulator of Ras-MAPK pathway of receptor tyrosine kinases, was induced by TCR signal and inhibited TCR signaling in CD4+ T cell clones. In this study, we addressed the mechanism underlying Sprouty1 inhibition of ... [more]

J. Immunol. Dec. 01, 2009; 183(11);7178-86 [Pubmed: 19915061]

Throughput

  • Low Throughput

Curated By

  • BioGRID