MAP3K5
Gene Ontology Biological Process
- JNK cascade [ISO]
- MAPK cascade [IDA, ISO]
- activation of MAPK activity [IDA]
- activation of MAPKK activity [IDA, ISO]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- cellular response to hydrogen peroxide [ISO]
- cellular response to reactive nitrogen species [IMP]
- intrinsic apoptotic signaling pathway in response to oxidative stress [IDA, ISO]
- positive regulation of apoptotic process [IDA, ISO]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- positive regulation of neuron death [ISO]
- positive regulation of protein phosphorylation [ISO]
- programmed necrotic cell death [IMP]
- protein phosphorylation [IDA, ISO]
- regulation of cell death [IGI]
Gene Ontology Molecular Function- ATP binding [ISO]
- MAP kinase kinase kinase activity [IDA, ISO]
- cysteine-type endopeptidase activator activity involved in apoptotic process [ISO]
- magnesium ion binding [ISO]
- protein binding [IPI]
- protein homodimerization activity [ISO]
- protein kinase activity [ISO]
- protein kinase binding [IPI]
- protein phosphatase binding [ISO]
- ATP binding [ISO]
- MAP kinase kinase kinase activity [IDA, ISO]
- cysteine-type endopeptidase activator activity involved in apoptotic process [ISO]
- magnesium ion binding [ISO]
- protein binding [IPI]
- protein homodimerization activity [ISO]
- protein kinase activity [ISO]
- protein kinase binding [IPI]
- protein phosphatase binding [ISO]
Gene Ontology Cellular Component
PARK7
Gene Ontology Biological Process
- adult locomotory behavior [IMP]
- cell proliferation [TAS]
- cellular response to hydrogen peroxide [IMP]
- cellular response to oxidative stress [IMP]
- cellular response to reactive oxygen species [IMP]
- dopamine uptake involved in synaptic transmission [IMP]
- hydrogen peroxide metabolic process [IMP]
- membrane depolarization [IMP]
- membrane hyperpolarization [IMP]
- mitochondrion organization [IMP]
- negative regulation of cell death [IMP]
- oxidation-reduction process [IMP]
- positive regulation of oxidative phosphorylation uncoupler activity [IMP]
- positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway [IMP]
- positive regulation of reactive oxygen species biosynthetic process [IDA]
- protein stabilization [IMP]
- regulation of inflammatory response [IMP]
- response to hydrogen peroxide [IDA, IMP]
- synaptic transmission, dopaminergic [IMP]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Structural determinants of the C-terminal helix-kink-helix motif essential for protein stability and survival promoting activity of DJ-1.
Mutations in the PARK7 gene encoding DJ-1 cause autosomal recessive Parkinson disease. The most deleterious point mutation is the L166P substitution, which resides in a structure motif comprising two alpha-helices (G and H) separated by a kink. Here we subjected the C-terminal helix-kink-helix motif to systematic site-directed mutagenesis, introducing helix-incompatible proline residues as well as conservative substitutions into the helical ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 8.
Curated By
- BioGRID