PREY

PSMC5

S8, SUG-1, SUG1, TBP10, TRIP1, p45, p45/SUG
proteasome (prosome, macropain) 26S subunit, ATPase, 5
GO Process (25)
GO Function (5)
GO Component (10)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Misfolding of proteins with a polyglutamine expansion is facilitated by proteasomal chaperones.

Rousseau E, Kojima R, Hoffner G, Djian P, Bertolotti A

Deposition of misfolded proteins with a polyglutamine expansion is a hallmark of Huntington disease and other neurodegenerative disorders. Impairment of the proteolytic function of the proteasome has been reported to be both a cause and a consequence of polyglutamine accumulation. Here we found that the proteasomal chaperones that unfold proteins to be degraded by the proteasome but also have non-proteolytic ... [more]

J. Biol. Chem. Jan. 16, 2009; 284(3);1917-29 [Pubmed: 18986984]

Throughput

  • Low Throughput

Additional Notes

  • source of proteins not clear

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PSMC5 HTT
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
727626

Curated By

  • BioGRID