An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The SH2/SH3 adaptor Grb4 transduces B-ephrin reverse signals.

Cowan CA, Henkemeyer M

Bidirectional signals mediated by membrane-anchored ephrins and Eph receptor tyrosine kinases have important functions in cell-cell recognition events, including those that occur during axon pathfinding and hindbrain segmentation. The reverse signal that is transduced into B-ephrin-expressing cells is thought to involve tyrosine phosphorylation of the signal's short, conserved carboxy-terminal cytoplasmic domain. The Src-homology-2 (SH2) domain proteins that associate with activated ... [more]

Nature Sep. 13, 2001; 413(6852);174-9 [Pubmed: 11557983]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
EFNB1 NCK2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Cowan CA (2001)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

EFNB1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ephrin receptor binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

NCK2