BAIT

CRYAB

CMD1II, CRYA2, CTPP2, CTRCT16, HEL-S-101, HSPB5, MFM2
crystallin, alpha B
Alzheimer's Disease Project
UPS Project
Homo sapiens
PREY

CRYBA1

CRYB1, CTRCT10
crystallin, beta A1
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

Identification of interaction sites between human betaA3- and alphaA/alphaB-crystallins by mammalian two-hybrid and fluorescence resonance energy transfer acceptor photobleaching methods.

Gupta R, Srivastava OP

Our recent study has shown that betaA3-crystallin along with betaB1- and betaB2-crystallins were part of high molecular weight complex obtained from young, old, and cataractous lenses suggesting potential interactions between alpha- and beta-crystallins (Srivastava, O. P., Srivastava, K., and Chaves, J. M. (2008) Mol. Vis. 14, 1872-1885). To investigate this further, this study was carried out to determine the interaction ... [more]

J. Biol. Chem. Jul. 03, 2009; 284(27);18481-92 [Pubmed: 19401464]

Throughput

  • Low Throughput

Additional Notes

  • figure 6,7.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CRYAB CRYBA1
Co-localization
Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Low-BioGRID
727785
CRYAB CRYBA1
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
727783
CRYAB CRYBA1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
727787

Curated By

  • BioGRID