Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The ubiquitin E3 ligase LOSS OF GDU 2 is required for GLUTAMINE DUMPER 1- induced amino secretion in Arabidopsis.

Pratelli R, Guerra DD, Yu S, Wogulis M, Kraft E, Frommer WB, Callis J, Pilot G

Amino acids serve as transport forms for organic nitrogen in the plant and multiple transport steps are involved in cellular import and export. While the nature of the export mechanism is unknown, over-expression of GLUTAMINE DUMPER 1 (GDU1) in Arabidopsis thaliana led to increased amino acid export. To gain insight into GDU1's role, we searched for ethyl-methanesulfonate suppressor mutants and ... [more]

Unknown Jan. 30, 2012; 0(0); [Pubmed: 22291198]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
GDU2 AT5G03200	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Pratelli R (2012)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

AT5G03200

Gene Ontology Molecular Function

ubiquitin-protein transferase activity [IDA]zinc ion binding [ISS]

Gene Ontology Cellular Component

GDU2

Gene Ontology Biological Process