PAK1
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- MAPK cascade [IDA]
- T cell costimulation [TAS]
- T cell receptor signaling pathway [TAS]
- actin cytoskeleton reorganization [IDA, IMP]
- axon guidance [TAS]
- branching morphogenesis of an epithelial tube [IMP]
- innate immune response [TAS]
- mitotic cell cycle [IBA]
- negative regulation of cell proliferation involved in contact inhibition [IMP]
- neuron projection morphogenesis [ISS]
- positive regulation of JUN kinase activity [IMP]
- positive regulation of intracellular estrogen receptor signaling pathway [IDA]
- positive regulation of peptidyl-serine phosphorylation [IDA]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of stress fiber assembly [IMP]
- protein autophosphorylation [IDA]
- protein phosphorylation [IDA]
- signal transduction by phosphorylation [IBA]
- wound healing [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
TGFBR1
Gene Ontology Biological Process
- activation of MAPKK activity [IDA]
- anterior/posterior pattern specification [ISS]
- artery morphogenesis [ISS]
- cell cycle arrest [TAS]
- cell motility [IMP]
- cellular response to transforming growth factor beta stimulus [IDA]
- collagen fibril organization [ISS]
- embryonic cranial skeleton morphogenesis [ISS]
- epithelial to mesenchymal transition [IDA]
- extracellular structure organization [TAS]
- germ cell migration [ISS]
- heart development [ISS]
- in utero embryonic development [ISS]
- kidney development [ISS]
- mesenchymal cell differentiation [TAS]
- negative regulation of chondrocyte differentiation [ISS]
- negative regulation of extrinsic apoptotic signaling pathway [IMP]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- neuron fate commitment [ISS]
- palate development [ISS]
- parathyroid gland development [ISS]
- pathway-restricted SMAD protein phosphorylation [IDA]
- peptidyl-serine phosphorylation [IDA]
- peptidyl-threonine phosphorylation [IDA]
- pharyngeal system development [ISS]
- positive regulation of SMAD protein import into nucleus [IDA]
- positive regulation of apoptotic signaling pathway [IDA]
- positive regulation of cell growth [IDA]
- positive regulation of cell proliferation [IMP]
- positive regulation of cellular component movement [IMP]
- positive regulation of pathway-restricted SMAD protein phosphorylation [IDA]
- positive regulation of protein kinase B signaling [IDA]
- positive regulation of transcription, DNA-templated [IDA]
- protein phosphorylation [IDA]
- regulation of protein ubiquitination [IDA]
- regulation of transcription, DNA-templated [IDA, IMP]
- response to cholesterol [IDA]
- signal transduction [IDA]
- skeletal system development [ISS]
- skeletal system morphogenesis [ISS]
- thymus development [ISS]
- transforming growth factor beta receptor signaling pathway [IC, IDA, IMP, TAS]
- wound healing [TAS]
Gene Ontology Molecular Function- ATP binding [IDA]
- I-SMAD binding [IPI]
- SMAD binding [IDA, IPI]
- growth factor binding [IPI]
- protein binding [IPI]
- protein kinase activity [IDA]
- protein serine/threonine kinase activity [IDA]
- transforming growth factor beta binding [IDA, IMP, IPI]
- transforming growth factor beta receptor activity, type I [IDA]
- transforming growth factor beta-activated receptor activity [IC, IDA, IMP]
- type II transforming growth factor beta receptor binding [IDA, IPI]
- ATP binding [IDA]
- I-SMAD binding [IPI]
- SMAD binding [IDA, IPI]
- growth factor binding [IPI]
- protein binding [IPI]
- protein kinase activity [IDA]
- protein serine/threonine kinase activity [IDA]
- transforming growth factor beta binding [IDA, IMP, IPI]
- transforming growth factor beta receptor activity, type I [IDA]
- transforming growth factor beta-activated receptor activity [IC, IDA, IMP]
- type II transforming growth factor beta receptor binding [IDA, IPI]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
High-throughput mapping of a dynamic signaling network in mammalian cells.
Signaling pathways transmit information through protein interaction networks that are dynamically regulated by complex extracellular cues. We developed LUMIER (for luminescence-based mammalian interactome mapping), an automated high-throughput technology, to map protein-protein interaction networks systematically in mammalian cells and applied it to the transforming growth factor-beta (TGFbeta) pathway. Analysis using self-organizing maps and k-means clustering identified links of the TGFbeta pathway ... [more]
Throughput
- Low Throughput
Additional Notes
- Iodinated [I-125] TGF-beta type I and type II receptors were visualized using autoradiography.
- Supplementary figure S6
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PAK1 TGFBR1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | 728219 |
Curated By
- BioGRID