An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The p97 ATPase associates with EEA1 to regulate the size of early endosomes.

Ramanathan HN, Ye Y

The AAA (ATPase-associated with various cellular activities) ATPase p97 acts on diverse substrate proteins to partake in various cellular processes such as membrane fusion and endoplasmic reticulum-associated degradation (ERAD). In membrane fusion, p97 is thought to function in analogy to the related ATPase NSF (N-ethylmaleimide-sensitive fusion protein), which promotes membrane fusion by disassembling a SNARE complex. In ERAD, p97 dislocates ... [more]

Cell Res. Feb. 01, 2012; 22(2);346-59 [Pubmed: 21556036]

Throughput

Low Throughput

Additional Notes

figure 7. oligomer formation by non-reducing gel.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
EEA1 EEA1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Liu X (2018)	High	-	BioGRID	-
EEA1 EEA1	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Dumas JJ (2001)	Low	-	BioGRID	-
EEA1 EEA1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Liu X (2018)	High	-	BioGRID	-
EEA1 EEA1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Callaghan J (1999)	Low	-	BioGRID	-
EEA1 EEA1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Callaghan J (1999)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

EEA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

1-phosphatidylinositol binding [IDA]GTP-dependent protein binding [IDA]calmodulin binding [NAS]protein binding [IPI]protein homodimerization activity [IDA]zinc ion binding [TAS]

Gene Ontology Cellular Component