ATG3
Gene Ontology Biological Process
Gene Ontology Molecular Function
ATG7
Gene Ontology Biological Process
- C-terminal protein lipidation [IBA]
- adult walking behavior [IMP]
- autophagy [IMP]
- cardiac muscle cell development [IMP]
- cellular amino acid metabolic process [IMP]
- cellular response to hyperoxia [ISO]
- cellular response to nitrogen starvation [IBA]
- cellular response to starvation [ISO]
- central nervous system neuron axonogenesis [IMP]
- cerebellar Purkinje cell layer development [IMP]
- cerebral cortex development [IMP]
- late nucleophagy [IBA]
- liver development [IMP]
- macroautophagy [IBA]
- membrane organization [IMP]
- mitochondrion degradation [IBA]
- mitochondrion organization [IMP]
- negative regulation of apoptotic process [IMP]
- negative regulation of histone H4-K16 acetylation [IMP]
- negative stranded viral RNA replication [IMP]
- neurological system process [IMP]
- neuron projection development [IMP]
- organelle organization [IMP]
- piecemeal microautophagy of nucleus [IBA]
- positive regulation of apoptotic process [ISO]
- positive regulation of autophagy [ISO]
- positive regulation of macroautophagy [ISO]
- positive regulation of mucus secretion [IMP]
- positive regulation of protein catabolic process [ISO]
- positive regulation of protein modification process [IDA, ISO]
- post-embryonic development [IMP]
- protein catabolic process [IMP]
- protein lipidation [ISO]
- protein modification by small protein conjugation [IMP]
- pyramidal neuron development [IMP]
- regulation of protein ubiquitination [IMP]
- response to starvation [IMP]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The FAP motif within human ATG7, an autophagy-related E1-like enzyme, is essential for the E2-substrate reaction of LC3 lipidation.
ATG7 is an autophagy-related E1-like enzyme that is essential for two ubiquitination-like reactions, ATG12-conjugation and LC3-lipidation. The existence of functional sequences at the amino-terminal region of human ATG7 remains uncertain. Mutational analyses of ATG7 revealed that both mutant ATG7ΔFAP lacking the FAP motif and ATG7FAPtoDDD, in which the Phe15-Ala16-Pro17 sequence was changed to Asp-Asp-Asp, could not complement defects in endogenous ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 4.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ATG3 ATG7 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 918054 | |
| ATG7 ATG3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 728515 | |
| ATG3 ATG7 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.887 | BioGRID | 2667020 | |
| ATG7 ATG3 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID