BAIT

PARK2

AR-JP, LPRS2, PDJ, PRKN, KB-152G3.1
parkin RBR E3 ubiquitin protein ligase
GO Process (58)
GO Function (22)
GO Component (12)

Gene Ontology Biological Process

Homo sapiens
PREY

UBE2J1

HSPC153, HSPC205, HSU93243, NCUBE-1, NCUBE1, UBC6, Ubc6p, CGI-76
ubiquitin-conjugating enzyme E2, J1
Homo sapiens

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

CHIP is associated with Parkin, a gene responsible for familial Parkinson's disease, and enhances its ubiquitin ligase activity.

Imai Y, Soda M, Hatakeyama S, Akagi T, Hashikawa T, Nakayama KI, Takahashi R

Unfolded Pael receptor (Pael-R) is a substrate of the E3 ubiquitin ligase Parkin. Accumulation of Pael-R in the endoplasmic reticulum (ER) of dopaminergic neurons induces ER stress leading to neurodegeneration. Here, we show that CHIP, Hsp70, Parkin, and Pael-R formed a complex in vitro and in vivo. The amount of CHIP in the complex was increased during ER stress. CHIP ... [more]

Mol. Cell Jul. 01, 2002; 10(1);55-67 [Pubmed: 12150907]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PARK2 UBE2J1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
PARK2 UBE2J1
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
PARK2 UBE2J1
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
PARK2 UBE2J1
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-

Curated By

  • BioGRID