An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

A novel route for F-box protein-mediated ubiquitination links CHIP to glycoprotein quality control.

Nelson RF, Glenn KA, Miller VM, Wen H, Paulson HL

In SCF (Skp1/Cullin/F-box protein) ubiquitin ligases, substrate specificity is conferred by a diverse array of F-box proteins. Only in fully assembled SCF complexes, it is believed, can substrates bound to F-box proteins become ubiquitinated. Here we show that Fbx2, a brain-enriched F-box protein implicated in the ubiquitination of glycoproteins discarded from the endoplasmic reticulum, binds the co-chaperone/ubiquitin ligase CHIP (C ... [more]

J. Biol. Chem. Jul. 21, 2006; 281(29);20242-51 [Pubmed: 16682404]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

FBXO2

Gene Ontology Biological Process

Gene Ontology Molecular Function

ubiquitin-protein transferase activity [TAS]

Gene Ontology Cellular Component

UBE2E1