BAIT

SH3RF1

POSH, RNF142, SH3MD2

SH3 domain containing ring finger 1

GO Process (3)

GO Function (1)

GO Component (1)

Gene Ontology Biological Process

negative regulation of apoptotic process [IMP]

negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]

negative regulation of extrinsic apoptotic signaling pathway [IMP]

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

CRISPR Database HGNC Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl

Homo sapiens

PREY

UBE2D1

E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A

ubiquitin-conjugating enzyme E2D 1

Cell Cycle - Senescence Project

GO Process (24)

GO Function (3)

GO Component (5)

Gene Ontology Biological Process

BMP signaling pathway [TAS]

MyD88-independent toll-like receptor signaling pathway [TAS]

TRIF-dependent toll-like receptor signaling pathway [TAS]

anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [TAS]

cellular response to hypoxia [TAS]

gene expression [TAS]

innate immune response [TAS]

mitotic cell cycle [TAS]

mitotic spindle assembly checkpoint [TAS]

negative regulation of transcription from RNA polymerase II promoter [TAS]

negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]

positive regulation of protein ubiquitination [IDA]

positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]

protein K48-linked ubiquitination [IDA]

protein polyubiquitination [IDA]

regulation of transcription from RNA polymerase II promoter in response to hypoxia [TAS]

regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]

toll-like receptor 3 signaling pathway [TAS]

toll-like receptor 4 signaling pathway [TAS]

toll-like receptor signaling pathway [TAS]

transcription initiation from RNA polymerase II promoter [TAS]

transcription, DNA-templated [TAS]

transforming growth factor beta receptor signaling pathway [TAS]

ubiquitin-dependent protein catabolic process [IDA]

Gene Ontology Molecular Function

protein binding [IPI]
ubiquitin conjugating enzyme activity [IDA]
ubiquitin-protein transferase activity [IDA]

Gene Ontology Cellular Component

cytoplasm [IDA]

nucleoplasm [TAS]

protein complex [IDA]

ubiquitin ligase complex [IDA]

CRISPR Database HGNC Alliance of Genome Resources OMIM Entrez Gene RefSeq UniprotKB Ensembl

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

POSH stimulates the ubiquitination and the clathrin-independent endocytosis of ROMK1 channels.

Lin DH, Yue P, Pan CY, Sun P, Zhang X, Han Z, Roos M, Caplan M, Giebisch G, Wang WH

POSH (plenty of SH3) is a scaffold protein that has been shown to act as an E3 ubiquitin ligase. Here we report that POSH stimulates the ubiquitination of Kir1.1 (ROMK) and enhances the internalization of this potassium channel. Immunostaining reveals the expression of POSH in the renal cortical collecting duct. Immunoprecipitation of renal tissue lysate with ROMK antibody and glutathione ... [more]

J. Biol. Chem. Oct. 23, 2009; 284(43);29614-24 [Pubmed: 19710010]

Throughput

Low Throughput

Curated By

BioGRID