BAIT
PRNP
AA960666, AI325101, CD230, PrP, PrP, PrPC, PrPSc, Prn-i, Prn-p, Sinc, prP27-30, prP33-35C, RP23-401J24.1
prion protein
GO Process (19)
GO Function (7)
GO Component (10)
Gene Ontology Biological Process
- cellular copper ion homeostasis [TAS]
- cellular response to copper ion [ISO]
- cellular response to drug [IDA]
- learning or memory [ISO]
- negative regulation of T cell receptor signaling pathway [IMP]
- negative regulation of activated T cell proliferation [IMP]
- negative regulation of apoptotic process [IGI, ISO]
- negative regulation of calcineurin-NFAT signaling cascade [IMP]
- negative regulation of interferon-gamma production [IMP]
- negative regulation of interleukin-17 production [IMP]
- negative regulation of interleukin-2 production [IMP]
- negative regulation of protein phosphorylation [IMP]
- negative regulation of sequence-specific DNA binding transcription factor activity [IMP]
- nucleobase-containing compound metabolic process [TAS]
- regulation of potassium ion transmembrane transport [IGI]
- regulation of protein localization [IMP]
- response to cadmium ion [ISO]
- response to copper ion [ISO]
- response to oxidative stress [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
ATP2B2
D6Abb2e, Gena300, PMCA2, Tmy, dfw, jog, wms, wri
ATPase, Ca++ transporting, plasma membrane 2
GO Process (31)
GO Function (9)
GO Component (12)
Gene Ontology Biological Process
- ATP catabolic process [IMP]
- auditory receptor cell stereocilium organization [IMP]
- cGMP metabolic process [IMP]
- calcium ion export [ISO]
- calcium ion transmembrane transport [ISO]
- calcium ion transport [IMP, ISO]
- cell morphogenesis [IMP]
- cellular calcium ion homeostasis [IMP]
- cerebellar Purkinje cell differentiation [IMP]
- cerebellar Purkinje cell layer morphogenesis [IMP]
- cerebellar granule cell differentiation [IMP]
- cerebellum development [IMP]
- cochlea development [IMP]
- cytosolic calcium ion homeostasis [IMP, ISO]
- detection of mechanical stimulus involved in sensory perception of sound [IMP]
- inner ear development [IMP]
- inner ear morphogenesis [IMP]
- inner ear receptor cell differentiation [IMP]
- lactation [IMP]
- locomotion [IMP]
- locomotory behavior [IMP]
- neuromuscular process controlling balance [IMP]
- neuron differentiation [ISO]
- organelle organization [IMP]
- otolith mineralization [IMP]
- positive regulation of calcium ion transport [IMP]
- regulation of cell size [IMP]
- regulation of synaptic plasticity [IMP]
- sensory perception of sound [IGI, IMP, ISO]
- serotonin metabolic process [IMP]
- synapse organization [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Time-controlled transcardiac perfusion cross-linking for the study of protein interactions in complex tissues.
Because of their sensitivity to solubilizing detergents, membrane protein assemblies are difficult to study. We describe a protocol that covalently conserves protein interactions through time-controlled transcardiac perfusion cross-linking (tcTPC) before disruption of tissue integrity. To validate tcTPC for identifying protein-protein interactions, we established that tcTPC allowed stringent immunoaffinity purification of the gamma-secretase complex in high salt concentrations and detergents and ... [more]
Nat. Biotechnol. Jun. 01, 2004; 22(6);724-31 [Pubmed: 15146195]
Throughput
- Low Throughput
Curated By
- BioGRID