BAIT
PRNP
AA960666, AI325101, CD230, PrP, PrP, PrPC, PrPSc, Prn-i, Prn-p, Sinc, prP27-30, prP33-35C, RP23-401J24.1
prion protein
GO Process (19)
GO Function (7)
GO Component (10)
Gene Ontology Biological Process
- cellular copper ion homeostasis [TAS]
- cellular response to copper ion [ISO]
- cellular response to drug [IDA]
- learning or memory [ISO]
- negative regulation of T cell receptor signaling pathway [IMP]
- negative regulation of activated T cell proliferation [IMP]
- negative regulation of apoptotic process [IGI, ISO]
- negative regulation of calcineurin-NFAT signaling cascade [IMP]
- negative regulation of interferon-gamma production [IMP]
- negative regulation of interleukin-17 production [IMP]
- negative regulation of interleukin-2 production [IMP]
- negative regulation of protein phosphorylation [IMP]
- negative regulation of sequence-specific DNA binding transcription factor activity [IMP]
- nucleobase-containing compound metabolic process [TAS]
- regulation of potassium ion transmembrane transport [IGI]
- regulation of protein localization [IMP]
- response to cadmium ion [ISO]
- response to copper ion [ISO]
- response to oxidative stress [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
APOE
AI255918
apolipoprotein E
GO Process (84)
GO Function (19)
GO Component (28)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [ISO]
- N-methyl-D-aspartate receptor clustering [ISO]
- aging [ISO]
- alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor clustering [ISO]
- artery morphogenesis [IGI]
- axon regeneration [IGI]
- cGMP-mediated signaling [ISO]
- cardiovascular system development [IMP]
- cellular calcium ion homeostasis [IDA]
- cholesterol biosynthetic process [IBA]
- cholesterol catabolic process [IMP]
- cholesterol efflux [IDA, IMP, ISO]
- cholesterol homeostasis [IGI, IMP, ISO]
- cholesterol metabolic process [IMP, ISO]
- chylomicron remnant clearance [ISO]
- fatty acid homeostasis [ISO]
- high-density lipoprotein particle assembly [IBA, ISO]
- high-density lipoprotein particle clearance [ISO]
- high-density lipoprotein particle remodeling [ISO]
- lipid homeostasis [IMP]
- lipid metabolic process [IGI]
- lipid transport [ISO]
- lipoprotein biosynthetic process [IGI]
- lipoprotein catabolic process [IMP]
- lipoprotein metabolic process [IGI, IMP, ISO]
- long-chain fatty acid transport [ISO]
- low-density lipoprotein particle remodeling [IMP]
- maintenance of location in cell [IMP]
- negative regulation of MAP kinase activity [ISO]
- negative regulation of beta-amyloid formation [ISO]
- negative regulation of blood coagulation [ISO]
- negative regulation of blood vessel endothelial cell migration [ISO]
- negative regulation of cholesterol biosynthetic process [ISO]
- negative regulation of cholesterol efflux [ISO]
- negative regulation of dendritic spine development [ISO]
- negative regulation of dendritic spine maintenance [ISO]
- negative regulation of endothelial cell proliferation [ISO]
- negative regulation of inflammatory response [IGI]
- negative regulation of lipid biosynthetic process [ISO]
- negative regulation of lipid transport across blood brain barrier [ISO]
- negative regulation of neuron apoptotic process [IBA, ISO]
- negative regulation of neuron death [ISO]
- negative regulation of phospholipid efflux [ISO]
- negative regulation of platelet activation [ISO]
- negative regulation of postsynaptic membrane organization [ISO]
- negative regulation of presynaptic membrane organization [ISO]
- nitric oxide mediated signal transduction [ISO]
- phospholipid efflux [IBA, ISO]
- positive regulation of axon extension [ISO]
- positive regulation of beta-amyloid formation [ISO]
- positive regulation of cGMP biosynthetic process [ISO]
- positive regulation of catalytic activity [IMP]
- positive regulation of cholesterol efflux [ISO]
- positive regulation of cholesterol esterification [IMP, ISO]
- positive regulation of dendritic spine development [ISO]
- positive regulation of dendritic spine maintenance [ISO]
- positive regulation of lipid biosynthetic process [IBA, ISO]
- positive regulation of lipid transport across blood brain barrier [ISO]
- positive regulation of low-density lipoprotein particle receptor catabolic process [ISO]
- positive regulation of membrane protein ectodomain proteolysis [ISO]
- positive regulation of neurofibrillary tangle assembly [ISO]
- positive regulation of neuron death [ISO]
- positive regulation of nitric-oxide synthase activity [IBA, ISO]
- positive regulation of phospholipid efflux [ISO]
- positive regulation of postsynaptic membrane organization [ISO]
- positive regulation of presynaptic membrane organization [ISO]
- protein import [ISO]
- protein localization [IMP]
- receptor-mediated endocytosis [ISO]
- regulation of Cdc42 protein signal transduction [ISO]
- regulation of axon extension [IBA]
- regulation of beta-amyloid clearance [IBA, ISO]
- regulation of cholesterol transport [IBA]
- regulation of gene expression [IMP]
- regulation of neuron death [ISO]
- regulation of tau-protein kinase activity [ISO]
- response to dietary excess [IMP]
- response to oxidative stress [IMP]
- reverse cholesterol transport [IBA, ISO]
- triglyceride catabolic process [IBA]
- triglyceride metabolic process [ISO]
- vasodilation [IMP]
- very-low-density lipoprotein particle clearance [ISO]
- very-low-density lipoprotein particle remodeling [IMP, ISO]
Gene Ontology Molecular Function- antioxidant activity [ISO]
- beta-amyloid binding [IBA, ISO]
- cholesterol binding [IBA]
- cholesterol transporter activity [IDA]
- heparin binding [ISO]
- hydroxyapatite binding [ISO]
- identical protein binding [ISO]
- lipid binding [ISO]
- lipid transporter activity [ISO]
- lipoprotein particle binding [IDA]
- low-density lipoprotein particle receptor binding [IBA, ISO]
- metal chelating activity [ISO]
- phosphatidylcholine-sterol O-acyltransferase activator activity [IMP, ISO]
- phospholipid binding [IBA, ISO]
- protein binding [IPI]
- protein homodimerization activity [ISO]
- receptor binding [ISO]
- tau protein binding [ISO]
- very-low-density lipoprotein particle receptor binding [IBA, ISO]
- antioxidant activity [ISO]
- beta-amyloid binding [IBA, ISO]
- cholesterol binding [IBA]
- cholesterol transporter activity [IDA]
- heparin binding [ISO]
- hydroxyapatite binding [ISO]
- identical protein binding [ISO]
- lipid binding [ISO]
- lipid transporter activity [ISO]
- lipoprotein particle binding [IDA]
- low-density lipoprotein particle receptor binding [IBA, ISO]
- metal chelating activity [ISO]
- phosphatidylcholine-sterol O-acyltransferase activator activity [IMP, ISO]
- phospholipid binding [IBA, ISO]
- protein binding [IPI]
- protein homodimerization activity [ISO]
- receptor binding [ISO]
- tau protein binding [ISO]
- very-low-density lipoprotein particle receptor binding [IBA, ISO]
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- blood microparticle [IBA, ISO]
- cell [IDA]
- cell surface [ISO]
- chylomicron [IBA, ISO]
- cytoplasm [ISO]
- dendrite [ISO]
- discoidal high-density lipoprotein particle [ISO]
- early endosome [ISO]
- endoplasmic reticulum [ISO]
- endosome [ISO]
- extracellular matrix [ISO]
- extracellular space [IDA, ISO]
- extracellular vesicular exosome [ISO]
- extrinsic component of external side of plasma membrane [ISO]
- high-density lipoprotein particle [IBA, ISO]
- intermediate-density lipoprotein particle [IBA, ISO]
- late endosome [ISO]
- low-density lipoprotein particle [IDA, ISO]
- lysosome [ISO]
- membrane [ISO]
- microtubule [ISO]
- neuronal cell body [ISO]
- nuclear envelope [ISO]
- nucleus [ISO]
- plasma membrane [ISO]
- very-low-density lipoprotein particle [ISO]
- vesicle [ISO]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Time-controlled transcardiac perfusion cross-linking for the study of protein interactions in complex tissues.
Because of their sensitivity to solubilizing detergents, membrane protein assemblies are difficult to study. We describe a protocol that covalently conserves protein interactions through time-controlled transcardiac perfusion cross-linking (tcTPC) before disruption of tissue integrity. To validate tcTPC for identifying protein-protein interactions, we established that tcTPC allowed stringent immunoaffinity purification of the gamma-secretase complex in high salt concentrations and detergents and ... [more]
Nat. Biotechnol. Jun. 01, 2004; 22(6);724-31 [Pubmed: 15146195]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PRNP APOE | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.754 | BioGRID | 2666820 |
Curated By
- BioGRID