BAIT
APH1A
6530402N02Rik, APH-1a
anterior pharynx defective 1a homolog (C. elegans)
GO Process (8)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
- Notch receptor processing [IMP, ISO]
- amyloid precursor protein catabolic process [ISO]
- membrane protein ectodomain proteolysis [ISO]
- membrane protein intracellular domain proteolysis [ISO]
- metanephros development [IMP]
- positive regulation of catalytic activity [ISO]
- protein processing [ISA, ISO]
- proteolysis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
ATP1A3
Atpa-2
ATPase, Na+/K+ transporting, alpha 3 polypeptide
GO Process (12)
GO Function (3)
GO Component (12)
Gene Ontology Biological Process
- ATP catabolic process [ISO]
- adult locomotory behavior [IMP]
- cellular potassium ion homeostasis [ISO]
- cellular sodium ion homeostasis [ISO]
- ionotropic glutamate receptor signaling pathway [IMP]
- memory [IMP]
- potassium ion import [ISO]
- potassium ion transport [ISO]
- response to drug [IMP]
- sodium ion export from cell [ISO]
- sodium ion transport [ISO]
- visual learning [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Time-controlled transcardiac perfusion cross-linking for the study of protein interactions in complex tissues.
Because of their sensitivity to solubilizing detergents, membrane protein assemblies are difficult to study. We describe a protocol that covalently conserves protein interactions through time-controlled transcardiac perfusion cross-linking (tcTPC) before disruption of tissue integrity. To validate tcTPC for identifying protein-protein interactions, we established that tcTPC allowed stringent immunoaffinity purification of the gamma-secretase complex in high salt concentrations and detergents and ... [more]
Nat. Biotechnol. Jun. 01, 2004; 22(6);724-31 [Pubmed: 15146195]
Throughput
- Low Throughput
Curated By
- BioGRID