BAIT
APH1A
6530402N02Rik, APH-1a
anterior pharynx defective 1a homolog (C. elegans)
GO Process (8)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
- Notch receptor processing [IMP, ISO]
- amyloid precursor protein catabolic process [ISO]
- membrane protein ectodomain proteolysis [ISO]
- membrane protein intracellular domain proteolysis [ISO]
- metanephros development [IMP]
- positive regulation of catalytic activity [ISO]
- protein processing [ISA, ISO]
- proteolysis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
PLP1
DM20, Plp, jimpy, jp, msd, rsh, RP23-389M3.1
proteolipid protein (myelin) 1
GO Process (11)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
- astrocyte development [IGI]
- axon development [IGI]
- axon ensheathment [IMP]
- cell maturation [IMP]
- central nervous system myelination [IMP]
- glial cell differentiation [ISO]
- inflammatory response [IGI]
- integrin-mediated signaling pathway [ISO]
- long-chain fatty acid biosynthetic process [IMP]
- myelination [IGI, IMP, ISO, TAS]
- positive regulation of gene expression [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Time-controlled transcardiac perfusion cross-linking for the study of protein interactions in complex tissues.
Because of their sensitivity to solubilizing detergents, membrane protein assemblies are difficult to study. We describe a protocol that covalently conserves protein interactions through time-controlled transcardiac perfusion cross-linking (tcTPC) before disruption of tissue integrity. To validate tcTPC for identifying protein-protein interactions, we established that tcTPC allowed stringent immunoaffinity purification of the gamma-secretase complex in high salt concentrations and detergents and ... [more]
Nat. Biotechnol. Jun. 01, 2004; 22(6);724-31 [Pubmed: 15146195]
Throughput
- Low Throughput
Curated By
- BioGRID