An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Time-controlled transcardiac perfusion cross-linking for the study of protein interactions in complex tissues.

Schmitt-Ulms G, Hansen K, Liu J, Cowdrey C, Yang J, DeArmond SJ, Cohen FE, Prusiner SB, Baldwin MA

Because of their sensitivity to solubilizing detergents, membrane protein assemblies are difficult to study. We describe a protocol that covalently conserves protein interactions through time-controlled transcardiac perfusion cross-linking (tcTPC) before disruption of tissue integrity. To validate tcTPC for identifying protein-protein interactions, we established that tcTPC allowed stringent immunoaffinity purification of the gamma-secretase complex in high salt concentrations and detergents and ... [more]

Nat. Biotechnol. Jun. 01, 2004; 22(6);724-31 [Pubmed: 15146195]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

APH1B

Gene Ontology Biological Process

Gene Ontology Molecular Function

endopeptidase activity [IGI]

Gene Ontology Cellular Component

YWHAZ