BAIT
APH1B
2310057K14Rik, Aph1c
anterior pharynx defective 1b homolog (C. elegans)
GO Process (1)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
ATP1A1
Atpa-1, BC010319
ATPase, Na+/K+ transporting, alpha 1 polypeptide
GO Process (24)
GO Function (13)
GO Component (18)
Gene Ontology Biological Process
- ATP catabolic process [IMP]
- cellular potassium ion homeostasis [ISO]
- cellular response to mechanical stimulus [ISO]
- cellular response to steroid hormone stimulus [ISO]
- cellular sodium ion homeostasis [ISO]
- dephosphorylation [IMP]
- membrane hyperpolarization [ISO]
- membrane repolarization [ISO]
- negative regulation of glucocorticoid biosynthetic process [IMP]
- negative regulation of heart contraction [IMP]
- positive regulation of heart contraction [IMP]
- positive regulation of striated muscle contraction [IMP]
- potassium ion import [ISO]
- potassium ion transmembrane transport [IMP]
- potassium ion transport [ISO]
- regulation of blood pressure [IGI]
- regulation of cardiac muscle cell contraction [ISO]
- regulation of sodium ion transport [ISO]
- regulation of the force of heart contraction [IMP]
- response to drug [IMP]
- response to glycoside [IGI]
- sodium ion export from cell [ISO]
- sodium ion transmembrane transport [IMP, ISO]
- sodium ion transport [ISO]
Gene Ontology Molecular Function- ADP binding [ISO]
- ATP binding [ISO]
- ankyrin binding [ISO]
- chaperone binding [ISO]
- phosphatase activity [IMP]
- phosphatidylinositol 3-kinase binding [ISO]
- potassium ion binding [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein kinase binding [ISO]
- sodium ion binding [ISO]
- sodium:potassium-exchanging ATPase activity [IDA, IMP, ISO]
- steroid hormone binding [ISO]
- ADP binding [ISO]
- ATP binding [ISO]
- ankyrin binding [ISO]
- chaperone binding [ISO]
- phosphatase activity [IMP]
- phosphatidylinositol 3-kinase binding [ISO]
- potassium ion binding [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein kinase binding [ISO]
- sodium ion binding [ISO]
- sodium:potassium-exchanging ATPase activity [IDA, IMP, ISO]
- steroid hormone binding [ISO]
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- T-tubule [IDA, ISO]
- apical plasma membrane [IDA]
- basolateral plasma membrane [IDA]
- caveola [ISO]
- endoplasmic reticulum [ISO]
- endosome [ISO]
- extracellular vesicular exosome [ISO]
- intercalated disc [ISO]
- membrane [IDA, ISO]
- membrane raft [ISO]
- myelin sheath [IDA]
- neuronal postsynaptic density [IDA]
- plasma membrane [IDA, ISO]
- protein complex [ISO]
- sarcolemma [IDA, ISO]
- sodium:potassium-exchanging ATPase complex [ISO]
- vesicle [ISO]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Time-controlled transcardiac perfusion cross-linking for the study of protein interactions in complex tissues.
Because of their sensitivity to solubilizing detergents, membrane protein assemblies are difficult to study. We describe a protocol that covalently conserves protein interactions through time-controlled transcardiac perfusion cross-linking (tcTPC) before disruption of tissue integrity. To validate tcTPC for identifying protein-protein interactions, we established that tcTPC allowed stringent immunoaffinity purification of the gamma-secretase complex in high salt concentrations and detergents and ... [more]
Nat. Biotechnol. Jun. 01, 2004; 22(6);724-31 [Pubmed: 15146195]
Throughput
- Low Throughput
Curated By
- BioGRID