BAIT
APH1B
2310057K14Rik, Aph1c
anterior pharynx defective 1b homolog (C. elegans)
GO Process (1)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
ATP1B1
Atp4b, Atpb, Atpb-1, NKbeta1
ATPase, Na+/K+ transporting, beta 1 polypeptide
GO Process (26)
GO Function (5)
GO Component (11)
Gene Ontology Biological Process
- ATP catabolic process [ISO]
- blastocyst development [IMP]
- cardiac muscle contraction [IMP]
- cellular calcium ion homeostasis [IMP]
- cellular potassium ion homeostasis [ISO]
- cellular sodium ion homeostasis [ISO]
- membrane repolarization [ISO]
- metal ion transport [ISO]
- positive regulation of ATP catabolic process [ISO]
- positive regulation of ATPase activity [ISO]
- positive regulation of calcium:sodium antiporter activity [IMP]
- positive regulation of potassium ion import [ISO]
- positive regulation of potassium ion transmembrane transporter activity [ISO]
- positive regulation of sodium ion export from cell [ISO]
- potassium ion import [IC, ISO]
- potassium ion transport [ISO]
- protein localization to plasma membrane [ISO]
- protein stabilization [IMP, ISO]
- regulation of calcium ion transmembrane transport [IMP]
- regulation of cardiac muscle contraction by calcium ion signaling [IMP]
- regulation of gene expression [IMP]
- relaxation of cardiac muscle [IMP]
- response to hypoxia [ISO]
- sodium ion export from cell [IC, ISO]
- sodium ion transmembrane transport [ISO]
- sodium ion transport [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Time-controlled transcardiac perfusion cross-linking for the study of protein interactions in complex tissues.
Because of their sensitivity to solubilizing detergents, membrane protein assemblies are difficult to study. We describe a protocol that covalently conserves protein interactions through time-controlled transcardiac perfusion cross-linking (tcTPC) before disruption of tissue integrity. To validate tcTPC for identifying protein-protein interactions, we established that tcTPC allowed stringent immunoaffinity purification of the gamma-secretase complex in high salt concentrations and detergents and ... [more]
Nat. Biotechnol. Jun. 01, 2004; 22(6);724-31 [Pubmed: 15146195]
Throughput
- Low Throughput
Curated By
- BioGRID